This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pNB101
catalog :
169110
citations: 1
Reference
Bongaerts N, Edoo Z, Abukar A, Song X, Sosa Carrillo S, Haggenmueller S, et al. Low-cost anti-mycobacterial drug discovery using engineered E. coli. Nat Commun. 2022;13:3905 pubmed publisher
product information
Catalog Number :
169110
Product Name :
pNB101
article :
doi10.1038/s41467-022-31570-3
id28216301
pubmed_id35798732
bacterial resistance :
Kanamycin
cloning :
backbonepNB101
backbone_mutation
backbone_origin
backbone_size2400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
Synthetic Biology
growth notes :
Plasmid design adapted from Daniel R, Rubens JR, Sarpeshkar R, Lu TK. Synthetic analog computation in living cells. Nature. 2013 May;497(7451):619 23. Please visit https://doi.org/10.1101/2021.03.26.437171 for bioRxiv preprint.
growth strain :
Feedback-controlled AraC circuit
origin :
37
pi :
alt_names
b0064
cloning
clone_methodRestriction Enzyme
cloning_site_3unknown
cloning_site_5unknown
promoter
sequencing_primer_3
sequencing_primer_5unknown
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesb0064, ECK0065
genearaC
id944780
genbank_ids
mutationCodon optimized for E. coli
namearaC
shRNA_sequence
size879
species
32644
Other
Escherichia coli K12 MG1655
tags
resistance markers :
5417
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA