pDestTol2CG2-mnx1:mCherry-PA-Rac1 | Addgene 169044 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pDestTol2CG2-mnx1:mCherry-PA-Rac1

catalog :

169044

citations: 1

Reference
Harris J, Wang A, Boulanger Weill J, Santoriello C, Foianini S, Lichtman J, et al. Long-Range Optogenetic Control of Axon Guidance Overcomes Developmental Boundaries and Defects. Dev Cell. 2020;53:577-588.e7 pubmed publisher

product information

Catalog Number :

169044

Product Name :

pDestTol2CG2-mnx1:mCherry-PA-Rac1

article :

doi	10.1016/j.devcel.2020.05.009
id	28216334
pubmed_id	32516597

bacterial resistance :

Ampicillin

cloning :

backbone

pDestTol2CG2

backbone_mutation

backbone_origin

Chi-Bin Chien lab

backbone_size

7796

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Destination transgenesis vector for expressing mCherry fused with an optimized photoactivatable Rac1 protein in zebrafish spinal motor neurons using 3 tandem repeats of the mnx1 enhancer.

origin :

pi :

alt_names

Photoactivatable Rac1

LOV(Leu404-Leu546)-(L514K-L531K)-Rac1(Ile4)-Q61L/E91H/N92H

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	3xMnx1
sequencing_primer_3	CCACTTTGTACAAGAAAGCTGGGT
sequencing_primer_5	GTTTGTACAAAAAAGCAGGC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MIG5, MRD48, Rac-1, TC-25, p21-Rac1
gene	RAC1
id	5879

genbank_ids

AB451363

mutation

Rac1 starts at I4 and contains mutations Q61L, E91H and N92H (results in the photoactivatable analogue of Rac1). L514K, L531K mutations in LOV domain reduce background activation.

name

PA-Rac-1

shRNA_sequence

size

1005

species

9606

Homo sapiens

32644

Other

Avena sativa (oat)