This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
p5E-3x125bp-mnx1
catalog :
169043
citations: 1
Reference
Harris J, Wang A, Boulanger Weill J, Santoriello C, Foianini S, Lichtman J, et al. Long-Range Optogenetic Control of Axon Guidance Overcomes Developmental Boundaries and Defects. Dev Cell. 2020;53:577-588.e7 pubmed publisher
product information
Catalog Number :
169043
Product Name :
p5E-3x125bp-mnx1
article :
doi10.1016/j.devcel.2020.05.009
id28216334
pubmed_id32516597
bacterial resistance :
Kanamycin
cloning :
backbonep5E
backbone_mutation
backbone_originChi-Bin Chien lab
backbone_size2663
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Gateway Entry Cloning Vector
growth notes :
See the following paper for cloning details of the 3x 125bp mnx1 enhancer: Zelenchuk, T.A. and Brus s, J.L. (2011), In Vivo labeling of zebrafish motor neurons using an mnx1 enhancer and Gal4/UAS. Genesis, 49: 546-554. https://doi.org/10.1002/dvg.20766
growth strain :
5' entry vector for Gateway cloning for expression in spinal motor neurons using 3 tandem repeats of the zebrafish mnx1 enhancer.
origin :
30
pi :
alt_names
Three tandem repeats of zebrafish mnx1 enhancer
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5XhoI
promoter3xMnx1
sequencing_primer_3CATCAGAGATTTTGAGACAC
sequencing_primer_5GTAAAACGACGGCCAG
site_3_destroyed1
site_5_destroyed
entrez_gene
aliaseshlxb, hlxb9, zgc:112174
genemnx1
id405399
genbank_ids
mutation
nameMnx1 enhancer (3x)
shRNA_sequence
size411
species
7955
Danio rerio
tags
resistance markers :
5520
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA