This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pME-mCherry-PA-Rac1
catalog :
169042
citations: 1
Reference
Harris J, Wang A, Boulanger Weill J, Santoriello C, Foianini S, Lichtman J, et al. Long-Range Optogenetic Control of Axon Guidance Overcomes Developmental Boundaries and Defects. Dev Cell. 2020;53:577-588.e7 pubmed publisher
product information
Catalog Number :
169042
Product Name :
pME-mCherry-PA-Rac1
article :
doi10.1016/j.devcel.2020.05.009
id28216334
pubmed_id32516597
bacterial resistance :
Kanamycin
cloning :
backbonepENTR/D-TOPO
backbone_mutation
backbone_originThermoFischer
backbone_size2709
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Gateway Entry Cloning Vector
growth strain :
Middle entry vector for Gateway cloning that contains an mCherry fused with an optimized photoactivatable Rac1 gene insert
origin :
30
pi :
alt_names
Photoactivatable Rac1
LOV(Leu404-Leu546)-(L514K-L531K)-Rac1(Ile4)-Q61L/E91H/N92H
cloning
clone_methodTOPO Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3CATCAGAGATTTTGAGACAC
sequencing_primer_5GTAAAACGACGGCCAG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMIG5, MRD48, Rac-1, TC-25, p21-Rac1
geneRAC1
id5879
genbank_ids
AB451363
mutationRac1 starts at I4 and contains mutations Q61L, E91H and N92H (results in the photoactivatable analogue of Rac1). L514K, L531K mutations in LOV domain reduce background activation.
namePA-Rac-1
shRNA_sequence
size1005
species
9606
Homo sapiens
32644
Other
Avena sativa (oat)
tags
locationN terminal on backbone
tagFused with mCherry
locationN terminal on backbone
tag6X His
plasmid copy :
Addgene 22027
resistance markers :
5520
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA