ZP45 Prickle-1 | Addgene 16897 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

ZP45 Prickle-1

catalog :

16897

product information

Catalog Number :

16897

Product Name :

ZP45 Prickle-1

article :

doi
id	1886
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pGEM-T easy

backbone_mutation

backbone_origin

Promega

backbone_size

3000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

Full length prickle-1 with 42bp of 5' UTR, cloned into pGEM-Teasy. The construct makes good in situ probes but does not translate in vitro due to an atg in the polylinker. To make antisense probe: cut with Nco1, transcribe with SP6. For the sense strand cut with Nde1 and transcribe with T7. Citation: Michael T. Veeman et al., Current Biology 13: 680-685 (2003).

origin :

pi :

alt_names

pk1

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	SP6
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	fk71b09, pk1, prickle1, wu:fk71b09
gene	prickle1a
id	368249

genbank_ids

mutation

name

Prickle-1

shRNA_sequence

size

species

7955

Danio rerio