This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBluescript Sp5
catalog :
16884
citations: 1
Reference
Tallafuss A, Wilm T, Crozatier M, Pfeffer P, Wassef M, Bally Cuif L. The zebrafish buttonhead-like factor Bts1 is an early regulator of pax2.1 expression during mid-hindbrain development. Development. 2001;128:4021-34 pubmed
product information
Catalog Number :
16884
Product Name :
pBluescript Sp5
article :
doi
id2091
pubmed_id11641225
bacterial resistance :
Ampicillin
cloning :
backbonepBluescript SK
backbone_mutation
backbone_originStratagene
backbone_size3000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Zebrafish sp5 (bts1) cDNA Full-length in pBluescript SK, cloned into EcoRI / XhoI. Useful for making antisense probe. Is the paralog of sp5-like (sp5l, clone 27-i2 from the Wnt8 upregulated microarray screen). The clone comes from library screening (library from Bruce Appel) and the library was constructed using EcoRI and XhoI linkers; The library was made into the Uni-ZAP XR Lambda vector, and from it Bluescript SK was excised.
origin :
37
pi :
alt_names
bts1
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5EcoRI
promoter
sequencing_primer_3T7
sequencing_primer_5SP6
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesbts1, fc39b01, wu:fc39b01
genesp5
id353154
genbank_ids
AF388363
mutation
namesp5
shRNA_sequence
size
species
7955
Danio rerio
tags
resistance markers :
19
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA