XE5 Xwnt-5A Hind RNA | Addgene 16872 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

XE5 Xwnt-5A Hind RNA

catalog :

16872

product information

Catalog Number :

16872

Product Name :

XE5 Xwnt-5A Hind RNA

article :

doi
id	1888
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

SP64T

backbone_mutation

backbone_origin

backbone_size

3000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

Xenopus Expression

growth notes :

Insert: oocyte cDNA from Melton lambda gt10 library. Insert size: 1526 bp. Transcription: linearize with Xba1 and transcribe with SP6. Sense: plasmid #3 on 5/22/90. Antisense: plasmid #2 on 5/22/90. Plasmid History: Constructed by Randall Moon. Date of Plasmid and by whom: Randall Moon, 4/9/90. Species of Sequence Origin: Xenopus. Notes for Use: lambda G6 Hind III RF in pGEM4 Blue (3/19/90) isolated and blunted into SP64T. Note: Hind III cuts 9aa 5' of a met which is used for translation, residing in putative leader sequence. This Hind III site is 13 aa 3' of actual initiation met. Removal of 5' UTR and the amino terminal 13 aa's yield a more active Xwnt, but the assumption is that using the second met, and the half leader sequence yield a wild type wnt after cleavage of the shortened leader. Citation: Moon et al., Development 119, 97 (1993)

origin :

pi :

alt_names

wnt5a

wnt

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5	Hind III RF blunted into Bgl II site
promoter
sequencing_primer_3
sequencing_primer_5	SP6
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	XELAEV_18003033mg, wnt-5A, wnt5a, wnt5a-B, wnt5a-a
gene	wnt5a.S
id	378689

genbank_ids

mutation

Starts with AA 14 (see comments)

name

Xwnt-5A Hind RNA

shRNA_sequence

size

1526

species

8355

Xenopus laevis