This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSP64T-dnWnt-11
catalog :
16857
citations: 1
| Reference |
|---|
Tada M, Smith J. Xwnt11 is a target of Xenopus Brachyury: regulation of gastrulation movements via Dishevelled, but not through the canonical Wnt pathway. Development. 2000;127:2227-38 pubmed
|
product information
Catalog Number :
16857
Product Name :
pSP64T-dnWnt-11
article :
| doi | |
| id | 2065 |
| pubmed_id | 10769246 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pSP64T | ||
| backbone_mutation | |||
| backbone_origin | Available at Addgene (#15030) | ||
| backbone_size | 3300 | ||
| promoter | |||
| sequencing_primer_3 | |||
| sequencing_primer_5 | |||
| vector_types |
|
growth notes :
A C-terminally truncated dominant-negative Xwnt11 construct (comprising amino acids 1-282) was generated according to Hoppler et al. (1996). PCR was carried out using pGEM-X9 (Ku and Melton, 1993) as a template and the primers 5 -CCCCCTCGAGAGTACCAATGGCTCCGACCCG-3 and 5 -TTGGAGATCTTTCAGCAGTAGTCAGGGGAAC-3 . The PCR product obtained was cloned into the XhoI and BglII sites of pSP64TXB. For injection, linearize with BamH1 and transcribe with SP6.
origin :
37
pi :
|
resistance markers :
| 488 |
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments