XE85 Delta Beta-catenin in GFP | Addgene 16835 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

XE85 Delta Beta-catenin in GFP

catalog :

16835

product information

Catalog Number :

16835

Product Name :

XE85 Delta Beta-catenin in GFP

article :

doi
id	1888
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

backbone_mutation

backbone_origin

backbone_size

6647

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

Xenopus Expression

growth notes :

To make RNA, cut with Not1 and transcribe with SP6. Plasmid History: Constructed by Randall Moon. Note For Use. Xenopus beta-catenin PCR amplified from st. 19 RNA. These PCR fragments have the first 138 bp after the AUG removed to eliminate a possible GSK-3 sequence. A new AUG has been inserted just after the Bam site. The mutant GFP of Hiem et al., inserted into Cla I site, followed by a STOP codon. This is no myc epitope. Reference: Moon, 1997, unpublished.

origin :

pi :

alt_names

beta catenin

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	T7
sequencing_primer_5	SP6
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	XELAEV_18031149mg, B-catenin, beta-catenin, ctnnb, ctnnb1, ctnnb1-a, ctnnb1-b
gene	ctnnb1.L
id	399274

genbank_ids

mutation

These PCR fragments have the first 138 bp after the AUG removed to eliminate a possible GSK-3 sequence. A new AUG has been inserted just after the Bam site. The mutant GFP of Hiem et al., inserted into Cla I site, followed by a STOP codon

name

Delta beta-catenin GFP

shRNA_sequence

size

species

8355

Xenopus laevis