This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
XE181 rat Frizzled-1-myc-pCD
catalog :
16819
product information
Catalog Number :
16819
Product Name :
XE181 rat Frizzled-1-myc-pCD
article :
| doi | |
| id | 1888 |
| pubmed_id |
bacterial resistance :
Ampicillin
cloning :
| backbone | pcS2+ | ||
| backbone_mutation | |||
| backbone_origin | |||
| backbone_size | 4095 | ||
| promoter | |||
| sequencing_primer_3 | |||
| sequencing_primer_5 | |||
| vector_types |
|
growth notes :
The original rat Frizzled-1 cDNA, tagged with a c-myc epitope at the Sty1 site of Frizzled1 (third extracellular loop), was cloned into CS2+. The CMV promoter was removed as a Sal-I-HindIII fragment, and the SV40 promoter fragment of the vector pCD-PS (as a Sal-I-SmaI fragment) was cloned in, destroying the HindIII site. As a result, this construct places the cDNA under the control of an SV40 promoter. Reference: The untagged part of the insert was published by Wang et al., JBC 271: 4468 (1996). The myc-tagged insert was made by Jeff Brown, and the remainder of this unpublished construct was generated by Julia Yang-Snyder.
origin :
37
pi :
|
resistance markers :
| 348 |
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments