XE239 Fz1-ER | Addgene 16816 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

XE239 Fz1-ER

catalog :

16816

citations: 1

Reference
Saito Diaz K, Benchabane H, Tiwari A, Tian A, Li B, Thompson J, et al. APC Inhibits Ligand-Independent Wnt Signaling by the Clathrin Endocytic Pathway. Dev Cell. 2018;44:566-581.e8 pubmed publisher

product information

Catalog Number :

16816

Product Name :

XE239 Fz1-ER

article :

doi
id	1888
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pCS2+

backbone_mutation

backbone_origin

backbone_size

4095

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

Xenopus Expression

growth notes :

This is a fusion protein consisting of a FKBP domain at the amino terminus of rat Frizzled 1. The expression of the protein is under the control of a CMV promoter. Once expressed the fusion protein enters the endoplasmic reticulum, where it remains trapped (and acts as a dominant negative Frizzled by trapping endogenous Frizzleds) unless one adds proprietary Ariad small molecule reagents that bind to and unfold the FKBP domain, allowing proteolysis and then processing and membrane targeting of the Frizzled 1. Note that the Frizzleds interact in a homo and heteromeric manner. Notes for Use: Construct is 8251bp, made 9/16/02 by Ajamete, and also called Fz1-ER/AKA pC4FZ1agg. Reference: A. Kaykas et al., 2004, Nature Cell Biology vol. 6, pp. 52-58.

origin :

pi :

alt_names

fz1

FZD1

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	SP6
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	Fzd1
id	58868

genbank_ids

mutation

name

Fz1-ER

shRNA_sequence

size

4156

species

10116

Rattus norvegicus