Gs(short)-CASE | Addgene 168124 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Gs(short)-CASE

catalog :

168124

citations: 1

Reference
Schihada H, Shekhani R, Schulte G. Quantitative assessment of constitutive G protein-coupled receptor activity with BRET-based G protein biosensors. Sci Signal. 2021;14:eabf1653 pubmed publisher

product information

Catalog Number :

168124

Product Name :

Gs(short)-CASE

article :

doi	10.1126/scisignal.abf1653
id	28216239
pubmed_id	34516756

bacterial resistance :

Kanamycin

cloning :

backbone

Clontech-style N1

backbone_mutation

backbone_origin

Clontech

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Luciferase

growth notes :

Please visit https://www.biorxiv.org/content/10.1101/2021.02.05.429900v1 for bioRxiv preprint.

growth strain :

Encodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).

origin :

pi :

alt_names

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	GNG1, HG3G1
gene	GNGT1
id	2792

aliases	AHO, C20orf45, GNAS1, GPSA, GSA, GSP, NESP, PITA3, POH, SCG6, SgVI
gene	GNAS
id	2778

aliases	HG2A, MDS, MRD42
gene	GNB1
id	2782

genbank_ids

mutation

NLuc is inserted at N136/V137 within GNAS

name

GNAS, GNB1, GNG1

shRNA_sequence

size

species

9606

Homo sapiens