This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Gi2-CASE
catalog :
168121
citations: 1
Reference
Schihada H, Shekhani R, Schulte G. Quantitative assessment of constitutive G protein-coupled receptor activity with BRET-based G protein biosensors. Sci Signal. 2021;14:eabf1653 pubmed publisher
product information
Catalog Number :
168121
Product Name :
Gi2-CASE
article :
doi10.1126/scisignal.abf1653
id28216239
pubmed_id34516756
bacterial resistance :
Kanamycin
cloning :
backboneClontech-style N1
backbone_mutation
backbone_originClontech
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Luciferase
growth notes :
Please visit https://www.biorxiv.org/content/10.1101/2021.02.05.429900v1 for bioRxiv preprint.
growth strain :
Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi2. Composed of the subunits G alpha i2 (GNAI2) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGIP, GNAI2B, HG1C, H_LUCA15.1, H_LUCA16.1
geneGNAI2
id2771
aliasesHG2A, MDS, MRD42
geneGNB1
id2782
aliasesHG3F1
geneGNG2
id54331
genbank_ids
mutationNLuc is inserted at C112/E115 within GNAI2
nameGNAI2, GNB1, GNG2
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagcpVenus on GNG2
resistance markers :
5393
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA