This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
AAVS1-EF1a-mTagRFP-T-CAAX
catalog :
167973
citations: 1
Reference
Sun D, Evans L, Perrone F, Sokleva V, Lim K, Rezakhani S, et al. A functional genetic toolbox for human tissue-derived organoids. elife. 2021;10: pubmed publisher
product information
Catalog Number :
167973
Product Name :
AAVS1-EF1a-mTagRFP-T-CAAX
article :
doi10.7554/eLife.67886
id28216212
pubmed_id34612202
bacterial resistance :
Ampicillin
cloning :
backbonepUC57
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
Other
Donor Template
growth notes :
CAG promoter was switched to EF1a promoter from AICSDP-42:AAVS1-mTagRFPT-CAAX (Addgene: Plasmid #107580) to reduce the insert size. Please visit https://www.biorxiv.org/content/10.1101/2020.05.04.076067v2 for bioRxiv preprint.
growth strain :
AAVS1 targeting repair template
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5HindIII
promoter
sequencing_primer_3TGTAAAACGACGGCCAGT
sequencing_primer_5CAGGAAACAGCTATGAC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAAVS1, LENG3, MBS85, p84, p85
genePPP1R12C
id54776
aliasesAAV
geneAAVS1
id17
genbank_ids
mutationhomology arms contain point mutations to disrupt crRNA binding sites used and point mutations specific to WTC genome
nameAAVS1
shRNA_sequence
size
species
9606
Homo sapiens
tags
location
tagmTagRFP-T-CAAX
resistance markers :
5394
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA