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product summary
company name :
Addgene
product type :
cDNA
product name :
XE127 Rfz1HA-CS2+
catalog :
16797
product information
Catalog Number :
16797
Product Name :
XE127 Rfz1HA-CS2+
article :
| doi | |
| id | 1888 |
| pubmed_id |
bacterial resistance :
Ampicillin
cloning :
| backbone | pCS2+ | ||
| backbone_mutation | |||
| backbone_origin | |||
| backbone_size | 4095 | ||
| promoter | |||
| sequencing_primer_3 | |||
| sequencing_primer_5 | |||
| vector_types |
|
growth notes :
Constructed by: Julia Yang-Snyder (subclone), Jeff Brown (tags), 4/19/96. Comments: Rfz1 from Henk Roelink. This sequence has a few differences. from the published sequence. Sites listed in Rfz1 are not a complete list. and I haven't searched through the CS2+ sequence to see if/where those sites. are present. HA tag version made by Jeff Brown (2/98-3/98) insert the epitope. into the StyI site (1904). The 3' Sty site is destroyed by the tags, but. the 5' site is inteact. Epitope can be detected in PBS/Formaldehyde fixed. tissue permeablized with 0.2% Triton. Frizzled tends to ppt out of SDS sample. buffer when boiled, so either warm samples to ~70 degrees Celsius for a. few minutes before loading samples or use a strong denaturing loading buffer. and incubate the samples at room temp for half and hour before loading. The StyI site is in the 3rd extracellular loop of the protein.
origin :
37
pi :
|
resistance markers :
| 348 |
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments