XE43 Xdsh-GFP | Addgene 16788 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

XE43 Xdsh-GFP

catalog :

16788

citations: 3

Reference
America M, Bostaille N, Eubelen M, Martin M, Stainier D, Vanhollebeke B. An integrated model for Gpr124 function in Wnt7a/b signaling among vertebrates. Cell Rep. 2022;39:110902 pubmed publisher
Eubelen M, Bostaille N, Cabochette P, Gauquier A, Tebabi P, Dumitru A, et al. A molecular mechanism for Wnt ligand-specific signaling. Science. 2018;361: pubmed publisher
Shnitsar I, Bashkurov M, Masson G, Ogunjimi A, Mosessian S, Cabeza E, et al. PTEN regulates cilia through Dishevelled. Nat Commun. 2015;6:8388 pubmed publisher

product information

Catalog Number :

16788

Product Name :

XE43 Xdsh-GFP

article :

doi
id	1888
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pCS2+

backbone_mutation

backbone_origin

backbone_size

4095

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

Xenopus Expression

growth notes :

Transcription: linearize with Not I and transcribe with SP6. Plasmid History: Constructed by Randall T. Moon. Notes for Use: Xdsh of S. Sokol with GFP of Heim et al., Nature 1995, cloned at the carboxy terminal end, followed by a translation stop codon. Published reference: Yang-Snyder, J.A. et al. Curr. Biol. 6:1302-1306, 1996. Addgene QC found 2 point mutations, T511A and T717S, that do not affect function.

origin :

pi :

alt_names

Xdsh

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	SP6
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	XELAEV_18019661mg, dishevelled, dsh, dvl, dvl2, xdd1, xdsh
gene	dvl2.L
id	399301

genbank_ids

mutation

name

Xdsh-GFP

shRNA_sequence

size

species

8355

Xenopus laevis