This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pInducer(p20) x mKate2-NLS-P2A-HRas(N17)_ires-puro
catalog :
167832
citations: 1
Reference
Ponsioen B, Post J, Buissant des Amorie J, Laskaris D, van Ineveld R, Kersten S, et al. Quantifying single-cell ERK dynamics in colorectal cancer organoids reveals EGFR as an amplifier of oncogenic MAPK pathway signalling. Nat Cell Biol. 2021;23:377-390 pubmed publisher
product information
Catalog Number :
167832
Product Name :
pInducer(p20) x mKate2-NLS-P2A-HRas(N17)_ires-puro
article :
doi10.1038/s41556-021-00654-5
id28216201
pubmed_id33795873
bacterial resistance :
Ampicillin
cloning :
backbonepInducer20
backbone_mutation
backbone_originMeerbey et al., PNAS
backbone_size5900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Inducible expression of dominant negative HRas(N17) and P2A-coupled reporter mKate2-NLS
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterUbc, TRE
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesC-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras
geneHRAS
id3265
genbank_ids
mutationS17N
nameHRAS(N17)
shRNA_sequence
size570
species
9606
Homo sapiens
tags
resistance markers :
5084
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA