Venus-30aa-Noxa-4E-pEGFP-C1 | Addgene 167529 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

Venus-30aa-Noxa-4E-pEGFP-C1

catalog :

167529

product information

Catalog Number :

167529

Product Name :

Venus-30aa-Noxa-4E-pEGFP-C1

article :

doi
id	28216035
pubmed_id

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-C1

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

. Linked by a 30 amino acid (aa) linker. Noxa BH3 region has hydrophobic positions H1-H4 mutated to glutamic acid (E), referred to as BH3-4E mutation, BH3 mutations: C25E, L29E, F32E, L36E. When compared with EYFP, the Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an A207K, F224R and L232H mutations to make it monomeric. An additional A164V mutation in Venus occurred in this construct, which does not appear to affect fluorescence of the protein.
MPGKKARKNAQPSPARAPAELEVECATQLRRFGDKLNFR
QKLLNLISKLFCSGT
The Noxa sequence aligns with phorbol-12-myristate-13-acetate-induced protien 1 isoform 6 [Homo sapiens] (NCBI Reference Sequence: NM_021127.3) Venus fused to the N-terminus of human Noxa amino acid sequence:

growth strain :

To increase the length of the linker between Venus and Noxa-4E proteins

origin :

pi :

alt_names

Noxa

cloning

clone_method	Restriction Enzyme
cloning_site_3	Unknown
cloning_site_5	Unknown
promoter	CMV
sequencing_primer_3	TCGCCCTTTGACGTTGGAGTCCAC
sequencing_primer_5	TGACGCAAATGGGCGGTAGG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	APR, NOXA
gene	PMAIP1
id	5366

genbank_ids

mutation

C25E, L29E, F32E, L36E

name

PMA-induced protein 1

shRNA_sequence

size

species

9606

Homo sapiens