This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSpCas9nucl-T2A-mCherry - BAKgRNA1- BAKgRNA2
catalog :
167295
citations: 1
Reference
Crossley M, Song C, Bocek M, Choi J, Kousorous J, Sathirachinda A, et al. R-loop-derived cytoplasmic RNA-DNA hybrids activate an immune response. Nature. 2023;613:187-194 pubmed publisher
product information
Catalog Number :
167295
Product Name :
pSpCas9nucl-T2A-mCherry - BAKgRNA1- BAKgRNA2
article :
doi10.1038/s41586-021-03269-w
id28216121
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonePX458
backbone_mutationInserted a second U6 gRNA expression cassette
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please visit https://www.biorxiv.org/content/10.1101/2020.01.31.929075v1 for bioRxiv preprint.
growth strain :
Plasmid encoding for 2 gRNAs targeting the human BAK gene and a CMV driven nuclease Cas9 followed by self-cleaving mCherry
origin :
37
pi :
alt_names
cloning
entrez_gene
aliasesBAK, BAK-LIKE, BCL2L7, CDN1
geneBAK1
id578
genbank_ids
mutation
nameBAK
shRNA_sequenceGCATGAAGTCGACCACGAAG, GGCCATGCTGGTAGACGTGT
size
species
9606
Homo sapiens
tags
resistance markers :
2711
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA