This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDONR221_SMAD2_HAe2_Bsd
catalog :
167166
citations: 1
Reference
Huang Z, Loewer A. Generating Somatic Knockout Cell Lines with CRISPR-Cas9 Technology to Investigate SMAD Signaling. Methods Mol Biol. 2022;2488:81-97 pubmed publisher
product information
Catalog Number :
167166
Product Name :
pDONR221_SMAD2_HAe2_Bsd
article :
doi
id28216085
pubmed_id
bacterial resistance :
Kanamycin
cloning :
backbonepDONR221
backbone_mutation
backbone_originInvitrogen
backbone_size2471
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Repair template for gene editing in human cells
growth strain :
Blasticidin selection cassette flanked by homology arms from human SMAD2 exon 2 for use in creating CRISPR-Cas9 mediated gene knockout.
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3M13 reverse
sequencing_primer_5M13 forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCHTD8, JV18, JV18-1, LDS6, MADH2, MADR2, hMAD-2, hSMAD2
geneSMAD2
id4087
genbank_ids
NG_029946.1
mutation
nameRepair template to knockout SMAD2 by inserting a blasticidin resistance cassette
shRNA_sequence
size2325
species
9606
Homo sapiens
tags
location
tagblasticidin
resistance markers :
5385
tags :
High Copy
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA