This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCas9CyMutL
catalog :
166981
citations: 2
Reference
Ellington A, Reisch C. Efficient and iterative retron-mediated in vivo recombineering in Escherichia coli. Synth Biol (Oxf). 2022;7:ysac007 pubmed publisher
Ellington A, Reisch C. Generating Single Nucleotide Point Mutations in E. coli with the No-SCAR System. Methods Mol Biol. 2022;2479:119-133 pubmed publisher
product information
Catalog Number :
166981
Product Name :
pCas9CyMutL
article :
doi10.1093/synbio/ysac007
id28216071
pubmed_id35673614
bacterial resistance :
Chloramphenicol
cloning :
backbonepCas9CR4
backbone_mutation
backbone_originChristopher Reisch
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
CRISPR
Synthetic Biology
growth strain :
Encodes aTc-inducible spCas9 and cumate-inducible MutLE32K for transient suppression of mismatch repair
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterpCymRC
sequencing_primer_3CACGACAGGTTTCCCGACTGGAAAGC
sequencing_primer_5CGCAGCTCTGGCTGCTTAAC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesb4170, ECK4166
genemutL
id948691
genbank_ids
mutationE32K
nameMutL
shRNA_sequence
size
species
tags
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterpLacI
sequencing_primer_3
sequencing_primer_5cctagggatatattccgcttcc
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameCymR
shRNA_sequence
size992
species
100
Synthetic
tags
plasmid copy :
CymR from pAJM.847 (item#108524; Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038)
resistance markers :
3959
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA