This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSMT3-FBF2 SS/R
catalog :
166976
citations: 1
Reference
Bhat V, McCann K, Wang Y, Fonseca D, Shukla T, Alexander J, et al. Engineering a conserved RNA regulatory protein repurposes its biological function in vivo. elife. 2019;8: pubmed publisher
product information
Catalog Number :
166976
Product Name :
pSMT3-FBF2 SS/R
article :
doi10.7554/eLife.43788
id28216058
pubmed_id30652968
bacterial resistance :
Kanamycin
cloning :
backbonepSMT3
backbone_mutation
backbone_originChris Lima
backbone_size5633
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Expresses FBF-2 with SS/R mutations in repeat 5 in E. coli for purification and crystallization studies
origin :
37
pi :
alt_names
FBF
cloning
clone_methodRestriction Enzyme
cloning_site_3Not1
cloning_site_5BamH1
promoterT7
sequencing_primer_3T7 term
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCELE_F21H12.5
genefbf-2
id174017
genbank_ids
mutationFBF-2 PUM RNA binding domain; amino acids 164-575; changed cysteine 363 to serine, changed glutamine 367 to serine
nameFBF-2
shRNA_sequence
size
species
6239
Caenorhabditis elegans
tags
locationN terminal on backbone
tagHis-SUMO
resistance markers :
187
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA