This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCS2+ Sizzled
catalog :
16688
citations: 1
Reference
Salic A, Kroll K, Evans L, Kirschner M. Sizzled: a secreted Xwnt8 antagonist expressed in the ventral marginal zone of Xenopus embryos. Development. 1997;124:4739-48 pubmed
product information
Catalog Number :
16688
Product Name :
pCS2+ Sizzled
article :
doi
id2103
pubmed_id9428410
bacterial resistance :
Ampicillin
cloning :
backbonepCS2+
backbone_mutation
backbone_origin
backbone_size4100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Xenopus Expression
growth notes :
This encodes a secreted antagonist of Xwnt-8. The entire open reading frame of sizzled was ampli ed by low-cycle number PCR using Pfu polymerase and cloned as a BamHI-XhoI fragment into pCS2+ .
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5BamHI
promoter
sequencing_primer_3EBV reverse
sequencing_primer_5SP6
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesXELAEV_18021029mg
geneszl
id398029
genbank_ids
mutation
nameSizzled
shRNA_sequence
size
species
8355
Xenopus laevis
tags
resistance markers :
256
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA