This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRS405 SEC66-VAC8-GFP
catalog :
166840
citations: 1
Reference
Gatica D, Wen X, Cheong H, Klionsky D. Vac8 determines phagophore assembly site vacuolar localization during nitrogen starvation-induced autophagy. Autophagy. 2021;17:1636-1648 pubmed publisher
product information
Catalog Number :
166840
Product Name :
pRS405 SEC66-VAC8-GFP
article :
doi10.1080/15548627.2020.1776474
id28216042
pubmed_id32508216
bacterial resistance :
Ampicillin
cloning :
backbonepRS405
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
Linearize with AflII
growth strain :
Expresses ER localized Vac8-GFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterVAC8 promoter
sequencing_primer_3M13R
sequencing_primer_5M13F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYEL013W, YEB3
geneVAC8
id856702
aliasesYBR171W, HSS1, KAR7, SEC71
geneSEC66
id852469
genbank_ids
mutationSEC66 transmembrane domain
nameVAC8
shRNA_sequence
size2544
species
4932
Saccharomyces cerevisiae
tags
locationC terminal on insert
tagGFP
locationN terminal on insert
tagSEC66 transmembrane domain
resistance markers :
1772
tags :
Unknown
terms :
LEU2
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA