This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Venus-7aa-Cb5-pEGFP-C1
catalog :
166764
product information
Catalog Number :
166764
Product Name :
Venus-7aa-Cb5-pEGFP-C1
article :
doi
id28216035
pubmed_id
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
KITTVESNSSWWTNWVIPAISALVVALMYRLYMAED amino acid targeting sequence, which we refer to as Cb5 , for endoplasmic reticulum targeting. 7aa indicates the 7 amino acid flexible linker region between Venus and the Cb5 targeting signal.
growth strain :
To target Venus to the Endoplasmic Reticulum. Cb5 tail anchor inserts into the membrane and Venus faces the cytosol. This serves as a collisional control in FLIM-FRET experiments.
origin :
37
pi :
alt_names
Venus-Cb5, V-Cb5
cloning
clone_methodRestriction Enzyme
cloning_site_3Unknown
cloning_site_5Unknown
promoterCMV
sequencing_primer_3TCGCCCTTTGACGTTGGAGTCCAC
sequencing_primer_5TGACGCAAATGGGCGGTAGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCyb5
geneCyb5a
id64001
genbank_ids
mutation
nameCb5 tail anchor
shRNA_sequence
size
species
10116
Rattus norvegicus
tags
locationN terminal on backbone
tagVenus
plasmid copy :
See gene entry for cytochrome b5 [Rattus norvegicus] , See NCBI Reference Sequence: NP_071581.1. Here we have used only the C-terminal tail anchor sequence.
resistance markers :
4132
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA