This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Venus-79aa-ActA-pEGFP-C1
catalog :
166763
product information
Catalog Number :
166763
Product Name :
Venus-79aa-ActA-pEGFP-C1
article :
doi
id28216035
pubmed_id
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
GSGLRSRAQASNSAVDARV amino acid targeting sequence, which we refer to as ActA , for the mitochondrial outer membrane. Pearson's correlation of 0.8-0.9 with mitotracker red signal in cells. 79aa indicates the 79 amino acid flexible linker region between Venus and the ActA targeting signal.
SGLRSRGSGLRSRAQASNSAVDARGSGLRSRAQASNSAV
DARGSGLRSRAQASNSAVDAR
growth strain :
To target Venus to the mitochondrial outer membrane. ActA tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.
origin :
37
pi :
alt_names
Venus-ActA, V-ActA
cloning
clone_methodRestriction Enzyme
cloning_site_3Unknown
cloning_site_5Unknown
promoterCMV
sequencing_primer_3TCGCCCTTTGACGTTGGAGTCCAC
sequencing_primer_5TGACGCAAATGGGCGGTAGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliaseslmo0204
geneactA
id987035
genbank_ids
mutation
nameActA tail anchor (Listeria monocytogenes)
shRNA_sequence
size
species
100
Synthetic
32644
Other
Listeria monocytogenes
tags
locationN terminal on backbone
tagVenus
plasmid copy :
See gene entry for actin assembly-inducing protein ActA (NCBI Reference Sequence: WP_110138194.1). Here we have used only the C-terminal tail anchor sequence of this protein.
resistance markers :
4132
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA