Venus-BimEL(Noxa)-pEGFP-C1 | Addgene 166761 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

Venus-BimEL(Noxa)-pEGFP-C1

catalog :

166761

citations: 1

Reference
Osterlund E, Hirmiz N, Pemberton J, Nougarède A, Liu Q, Leber B, et al. Efficacy and specificity of inhibitors of BCL-2 family protein interactions assessed by affinity measurements in live cells. Sci Adv. 2022;8:eabm7375 pubmed publisher

product information

Catalog Number :

166761

Product Name :

Venus-BimEL(Noxa)-pEGFP-C1

article :

doi	10.1126/sciadv.abm7375
id	28231273
pubmed_id	35442739

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-C1

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

BH3 region removed and inserted BH3 of Noxa protein. There is a K107E mutation in the Venus sequence of this plasmid. The K107E mutation is facing outwards in the B-Barrel structure of the Venus protein. Therefore, it is unlikely that this mutation would affect the chromophore within the -barrel. We have transfected this plasmid in BMK-DKO cells and did not observe aggregation. We also used this construct and observed Forster resonance energy transfer from mCerulean3 in the context of measuring interactions with two binding partners. Thus, this mutation does not appear to affect the Venus fluorophore.

growth strain :

To examine effect of swapping the BH3 region in full length BimEL with that of Noxa

origin :

pi :

alt_names

BimEL-Noxa, BimEL(Noxa BH3), VBimEL(Noxa), VBimEL(Noxa BH3)

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3	TCGCCCTTTGACGTTGGAGTCCAC
sequencing_primer_5	TGACGCAAATGGGCGGTAGG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	1500006F24Rik, Bim, Bod, bcl2-L-11
gene	Bcl2l11
id	12125

aliases	APR, NOXA
gene	PMAIP1
id	5366

genbank_ids

mutation

BH3 region removed and inserted BH3 of human Noxa protein

name

BimEL(Mus musculus)-(Noxa BH3)

shRNA_sequence

size

species

9606

Homo sapiens

10090

Mus musculus