Venus-BimEL(Bad)-pEGFP-C1 | Addgene 166758 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Venus-BimEL(Bad)-pEGFP-C1

catalog :

166758

citations: 2

Reference
Osterlund E, Hirmiz N, Pemberton J, Nougarède A, Liu Q, Leber B, et al. Efficacy and specificity of inhibitors of BCL-2 family protein interactions assessed by affinity measurements in live cells. Sci Adv. 2022;8:eabm7375 pubmed publisher
Liu Q, Osterlund E, Chi X, Pogmore J, Leber B, Andrews D. Bim escapes displacement by BH3-mimetic anti-cancer drugs by double-bolt locking both Bcl-XL and Bcl-2. elife. 2019;8: pubmed publisher

product information

Catalog Number :

166758

Product Name :

Venus-BimEL(Bad)-pEGFP-C1

article :

doi	10.7554/eLife.37689
id	28216034
pubmed_id	30860026

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-C1

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

BH3 region removed and inserted BH3 of Bad protein. Linker between Venus and BimEL(Bad) is 3 amino acids in length.

growth strain :

To examine effect of swapping the BH3 region in full length BimEL with that of Bad

origin :

pi :

alt_names

BimEL-Bad, BimEL(Bad BH3), VBimEL(Bad), VBimEL(Bad BH3)

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3	TCGCCCTTTGACGTTGGAGTCCAC
sequencing_primer_5	TGACGCAAATGGGCGGTAGG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	1500006F24Rik, Bim, Bod, bcl2-L-11
gene	Bcl2l11
id	12125

aliases	BBC2, BCL2L8
gene	BAD
id	572

genbank_ids

mutation

BH3 region removed and inserted BH3 of human Bad protein

name

BimEL(Mus musculus)-(Bad BH3)

shRNA_sequence

size

species

9606

Homo sapiens

10090

Mus musculus