This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
mCerulean3-Bcl-W-FUGW
catalog :
166751
citations: 1
Reference
Osterlund E, Hirmiz N, Pemberton J, Nougarède A, Liu Q, Leber B, et al. Efficacy and specificity of inhibitors of BCL-2 family protein interactions assessed by affinity measurements in live cells. Sci Adv. 2022;8:eabm7375 pubmed publisher
product information
Catalog Number :
166751
Product Name :
mCerulean3-Bcl-W-FUGW
article :
doi10.1126/sciadv.abm7375
id28231273
pubmed_id35442739
bacterial resistance :
Ampicillin
cloning :
backboneFUGW
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
growth notes :
3rd generation lentiviral transfer plasmid that can be used to create lentivirus, for hUbC-driven gene expression of mCerulean3-fused to the N-terminus of Bcl-W. There is a 7 aa linker between mCerulean3 and Bcl-W.
growth strain :
Express mCerulean3 fused to Bcl-2 family protein, Bcl-W, in a plasmid compatable for lentiviral infection. Used to make stable BMK-DKO cell lines.
origin :
37
pi :
alt_names
Bcl2 like protein 2 (Bcl2l2)
cloning
clone_methodRestriction Enzyme
cloning_site_3Unknown
cloning_site_5Unknown
promoterhuman ubiquitin C
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAW048834, Bcl-, Bcl-w, Gtrg, Gtrgal2, Gtro, Gtrosa41, bcl, bclw, c98
geneBcl2l2
id12050
genbank_ids
mutation
nameBcl-W
shRNA_sequence
size
species
10090
Mus musculus
tags
locationN terminal on backbone
tagmCerulean3
plasmid copy :
mCerulean3 received from Mark Rizzo PMID: 21479270 and cloned into this plasmid. Bcl-W gene sequence aligns with Mus musculus BCL2-like 2 (Bcl2l2), transcript variant X2, mRNA (NCBI Reference Sequence: XM_006518457.5). FUGW backbone from Addgene #14883, where EGFP was replaced by mCerulean3-Bcl-W.
resistance markers :
4132
tags :
High Copy
terms :
Zeocin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA