This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
mCerulean3-Bcl-XL-s2193
catalog :
166749
citations: 2
Reference
Osterlund E, Hirmiz N, Pemberton J, Nougarède A, Liu Q, Leber B, et al. Efficacy and specificity of inhibitors of BCL-2 family protein interactions assessed by affinity measurements in live cells. Sci Adv. 2022;8:eabm7375 pubmed publisher
Liu Q, Osterlund E, Chi X, Pogmore J, Leber B, Andrews D. Bim escapes displacement by BH3-mimetic anti-cancer drugs by double-bolt locking both Bcl-XL and Bcl-2. elife. 2019;8: pubmed publisher
product information
Catalog Number :
166749
Product Name :
mCerulean3-Bcl-XL-s2193
article :
doi10.7554/eLife.37689
id28216034
pubmed_id30860026
bacterial resistance :
Blasticidin
cloning :
backbones2193
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Transfection of this plasmid will express mCerulean3-fused to the N-terminus of Bcl-XL. There is a flexible, 7 amino acid linker SGLRSRE , between mCerulean3 and Bcl-XL. Selection with Blasticidin was used to generate BMK-DKO cells stably expressing this plasmid. Expression under control of Human ferritin promoter.
growth strain :
Express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-XL, in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.
origin :
37
pi :
alt_names
Bcl2 like protein 1 (Bcl2l1)
cloning
clone_methodRestriction Enzyme
cloning_site_3Unknown
cloning_site_5Unknown
promoterHuman ferritin
sequencing_primer_3TTACCCCTCTAGACCTGGAAAG
sequencing_primer_5CCTTGAGTTTTGAGCGGAGCTAA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBCL-XL/S, BCL2L, BCLX, Bcl-X, PPP1R52
geneBCL2L1
id598
genbank_ids
mutation
nameBcl-XL
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagmCerulean3
plasmid copy :
mCerulean3 received from Mark Rizzo PMID: 21479270 and cloned into this plasmid. BclXL sequence aligns with NCBI Reference Sequence: NM_001317919.2 of Homo sapiens BCL2 like 1 (BCL2L1), transcript variant 3, mRNA.
resistance markers :
4132
tags :
Low Copy
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA