Venus-Bad-4E-pEGFP-C1 | Addgene 166740 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Venus-Bad-4E-pEGFP-C1

catalog :

166740

citations: 2

Reference
Osterlund E, Hirmiz N, Pemberton J, Nougarède A, Liu Q, Leber B, et al. Efficacy and specificity of inhibitors of BCL-2 family protein interactions assessed by affinity measurements in live cells. Sci Adv. 2022;8:eabm7375 pubmed publisher
Liu Q, Osterlund E, Chi X, Pogmore J, Leber B, Andrews D. Bim escapes displacement by BH3-mimetic anti-cancer drugs by double-bolt locking both Bcl-XL and Bcl-2. elife. 2019;8: pubmed publisher

product information

Catalog Number :

166740

Product Name :

Venus-Bad-4E-pEGFP-C1

article :

doi	10.7554/eLife.37689
id	28216034
pubmed_id	30860026

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-C1

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Transfection of this plasmid will express VBad-4E (Venus fused to the N-terminus of the BH3-only protein, Bad-4E) in live cells. There is a flexible, 7 amino acid linker between Venus and Bad-4E proteins. Full length Bad protein with BH3 region hydrophobic positions H1-H4 mutated to glutamic acid (E), refered to as BH3-4E mutation, BH3 mutations: Y110E, L114E, M117E, F122E. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric.

growth strain :

Express Venus fused to the N-terminus of the Bcl-2 family protein, Bad with mutation in BH3 domain to disrupt binding anti-apoptotic proteins

origin :

pi :

alt_names

Bcl2 associated agonist of cell death, or Bcl2 like protein 8 (BCL2L8)

cloning

clone_method	Restriction Enzyme
cloning_site_3	Unknown
cloning_site_5	Unknown
promoter	CMV
sequencing_primer_3	TCGCCCTTTGACGTTGGAGTCCAC
sequencing_primer_5	TGACGCAAATGGGCGGTAGG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BBC2, BCL2L8
gene	BAD
id	572

genbank_ids

mutation

4 hydrophobic residues in the BH3 region mutated to glutamic acid: Y110E, L114E, M117E, F122E.

name

Bad-4E

shRNA_sequence

size

species

9606

Homo sapiens