Venus-Noxa-pEGFP-C1 | Addgene 166738 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Venus-Noxa-pEGFP-C1

catalog :

166738

citations: 1

Reference
Osterlund E, Hirmiz N, Pemberton J, Nougarède A, Liu Q, Leber B, et al. Efficacy and specificity of inhibitors of BCL-2 family protein interactions assessed by affinity measurements in live cells. Sci Adv. 2022;8:eabm7375 pubmed publisher

product information

Catalog Number :

166738

Product Name :

Venus-Noxa-pEGFP-C1

article :

doi	10.1126/sciadv.abm7375
id	28231273
pubmed_id	35442739

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-C1

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Transfection of this plasmid will express VNoxa (Venus fused to the N-terminus of the BH3-only protein, Noxa) in live cells. There is an 8 amino acid flexible linker between Venus and Noxa proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an A207K, F224R and L232H mutations to make it monomeric.

growth strain :

Express Venus fused to the N-terminus of the Bcl-2 family protein, Noxa

origin :

pi :

alt_names

PMA-induced protein 1

cloning

clone_method	Restriction Enzyme
cloning_site_3	Unknown
cloning_site_5	Unknown
promoter	CMV
sequencing_primer_3	TCGCCCTTTGACGTTGGAGTCCAC
sequencing_primer_5	TGACGCAAATGGGCGGTAGG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	APR, NOXA
gene	PMAIP1
id	5366

genbank_ids

mutation

name

Noxa

shRNA_sequence

size

species

9606

Homo sapiens