DICER KO vector | Addgene 16656 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

DICER KO vector

catalog :

16656

citations: 2

Reference
Anbarlou A, AkhavanRahnama M, Atashi A, Soleimani M, Arefian E, Gallinella G. Possible involvement of miRNAs in tropism of Parvovirus B19. Mol Biol Rep. 2016;43:175-81 pubmed publisher
Cummins J, He Y, Leary R, Pagliarini R, Diaz L, Sjoblom T, et al. The colorectal microRNAome. Proc Natl Acad Sci U S A. 2006;103:3687-92 pubmed

product information

Catalog Number :

16656

Product Name :

DICER KO vector

article :

doi	10.1073/pnas.0511155103
id	1880
pubmed_id	16505370

bacterial resistance :

Ampicillin

cloning :

backbone

pBluescript

backbone_mutation

backbone_origin

Stratagene

backbone_size

3000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Other

adeno-associated viral

growth notes :

The targeting construct pAAV-Neo-Dicer was made by PCR, by using bacterial artificial chromosome clone CITB 2240H23 (Invitrogen) as the template for the homology arms. A targeted insertion was made in exon 5, which is part of the helicase domain. See Author's Map and article for more information.

origin :

pi :

alt_names

Dicer

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	DCR1, Dicer, Dicer1e, GLOW, HERNA, K12H4.8-LIKE, MNG1, RMSE2, aviD
gene	DICER1
id	23405

genbank_ids

mutation

name

Dicer knock-out

shRNA_sequence

size

species

9606

Homo sapiens