pCI-neo Flag HAUSP | Addgene 16655 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCI-neo Flag HAUSP

catalog :

16655

citations: 16

Reference
Qiao H, Tian Y, Huo Y, Man H. Role of the DUB enzyme USP7 in dendritic arborization, neuronal migration, and autistic-like behaviors in mice. iScience. 2022;25:104595 pubmed publisher
Frey Y, Franz Wachtel M, Macek B, Olayioye M. Proteasomal turnover of the RhoGAP tumor suppressor DLC1 is regulated by HECTD1 and USP7. Sci Rep. 2022;12:5036 pubmed publisher
Sun Y, Chen J, Huang S, Su Y, Wang W, Agama K, et al. PARylation prevents the proteasomal degradation of topoisomerase I DNA-protein crosslinks and induces their deubiquitylation. Nat Commun. 2021;12:5010 pubmed publisher
Li C, Xia J, Franqui Machin R, Chen F, He Y, Ashby T, et al. TRIP13 modulates protein deubiquitination and accelerates tumor development and progression of B cell malignancies. J Clin Invest. 2021;131: pubmed publisher
Zhang T, Periz G, Lu Y, Wang J. USP7 regulates ALS-associated proteotoxicity and quality control through the NEDD4L-SMAD pathway. Proc Natl Acad Sci U S A. 2020;117:28114-28125 pubmed publisher
Kim J, Yang Y, Park K, Ge X, Xu R, Li N, et al. A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation. Nat Commun. 2020;11:2289 pubmed publisher
Franqui Machin R, Hao M, Bai H, Gu Z, Zhan X, Habelhah H, et al. Destabilizing NEK2 overcomes resistance to proteasome inhibition in multiple myeloma. J Clin Invest. 2018;: pubmed publisher
Wang X, Zhang Q, Wang Y, Zhuang H, Chen B. Clinical Significance of Ubiquitin Specific Protease 7 (USP7) in Predicting Prognosis of Hepatocellular Carcinoma and its Functional Mechanisms. Med Sci Monit. 2018;24:1742-1750 pubmed
Biswas K, Philip S, Yadav A, Martin B, Burkett S, Singh V, et al. BRE/BRCC45 regulates CDC25A stability by recruiting USP7 in response to DNA damage. Nat Commun. 2018;9:537 pubmed publisher
Lamberto I, Liu X, Seo H, Schauer N, Iacob R, Hu W, et al. Structure-Guided Development of a Potent and Selective Non-covalent Active-Site Inhibitor of USP7. Cell Chem Biol. 2017;24:1490-1500.e11 pubmed publisher
Mungamuri S, Qiao R, Yao S, Manfredi J, Gu W, Aaronson S. USP7 Enforces Heterochromatinization of p53 Target Promoters by Protecting SUV39H1 from MDM2-Mediated Degradation. Cell Rep. 2016;14:2528-37 pubmed publisher
Papp S, Huber A, Jordan S, Kriebs A, Nguyen M, Moresco J, et al. DNA damage shifts circadian clock time via Hausp-dependent Cry1 stabilization. elife. 2015;4: pubmed publisher
Zemp I, Lingner J. The shelterin component TPP1 is a binding partner and substrate for the deubiquitinating enzyme USP7. J Biol Chem. 2014;289:28595-606 pubmed publisher
Hall J, Tabata M, Rodgers J, Puigserver P. USP7 attenuates hepatic gluconeogenesis through modulation of FoxO1 gene promoter occupancy. Mol Endocrinol. 2014;28:912-24 pubmed publisher
Khoronenkova S, Dianova I, Parsons J, Dianov G. USP7/HAUSP stimulates repair of oxidative DNA lesions. Nucleic Acids Res. 2011;39:2604-9 pubmed publisher
Cummins J, Vogelstein B. HAUSP is required for p53 destabilization. Cell Cycle. 2004;3:689-92 pubmed

product information

Catalog Number :

16655

Product Name :

pCI-neo Flag HAUSP

article :

doi	10.4161/cc.3.6.924
id	1879
pubmed_id	15118411

bacterial resistance :

Ampicillin

cloning :

backbone

pCI-Neo

backbone_mutation

backbone_origin

Promega

backbone_size

5472

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

A fragment of human HAUSP cDNA including the entire ORF was cloned into pCI-neo at the NheI and XbaI sites. (Please note that the XbaI site is blocked by overlapping methylation). A sequence including the Flag tag was added to one of the primers so that the encoded protein contains a Flag tag at the N-terminus.

origin :

pi :

alt_names

USP7

ubiquitin specific peptidase 7

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	NheI
promoter	CMV
sequencing_primer_3	EBV-rev
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	C16DELp13.2, DEL16P13.2, HAFOUS, HAUSP, TEF1
gene	USP7
id	7874

genbank_ids

mutation

name

HAUSP

shRNA_sequence

size

species

9606

Homo sapiens