pCMV-Myc CDC4 WT* | Addgene 16652 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCMV-Myc CDC4 WT*

catalog :

16652

citations: 3

Reference
Liu J, Shi H, Li X, Chen G, Larsson C, Lui W. miR‑223‑3p regulates cell growth and apoptosis via FBXW7 suggesting an oncogenic role in human testicular germ cell tumors. Int J Oncol. 2017;50:356-364 pubmed publisher
Deng W, Vanderbilt D, Lin C, Martin K, Brundage K, Ruppert J. SOX9 inhibits Î²-TrCP-mediated protein degradation to promote nuclear GLI1 expression and cancer stem cell properties. J Cell Sci. 2015;128:1123-38 pubmed publisher
Rajagopalan H, Jallepalli P, Rago C, Velculescu V, Kinzler K, Vogelstein B, et al. Inactivation of hCDC4 can cause chromosomal instability. Nature. 2004;428:77-81 pubmed

product information

Catalog Number :

16652

Product Name :

pCMV-Myc CDC4 WT*

article :

doi	10.1038/nature02313
id	1878
pubmed_id	14999283

bacterial resistance :

Ampicillin

cloning :

backbone

pCMV-Myc

backbone_mutation

backbone_origin

Clontech

backbone_size

3800

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

A 1.5kb fragment of human CDC4 cDNA (nucleotides 374-1874), which encompasses the entire ORF, was cloned into pCMV-Myc. Addgene's quality control sequence suggests there are some unexpected mutations in the gene. The effect these mutations have on function is unknown, but this is the plasmid used in the Vogelstein article, so it should behave as reported.

origin :

pi :

alt_names

Archipelago

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	BglII
promoter
sequencing_primer_3	EBV rev
sequencing_primer_5	n/a
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AGO, CDC4, DEDHIL, FBW6, FBW7, FBX30, FBXO30, FBXW6, SEL-10, SEL10, hAgo, hCdc4
gene	FBXW7
id	55294

genbank_ids

mutation

*Note that this insert is only WT relative to the mutant version (www.addgene.org/16653). This is not full length CDC4. See comments and paper for specifics.

name

CDC4

shRNA_sequence

size

1500

species

9606

Homo sapiens