PIK3CA-WT (pFastBac) | Addgene 16644 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

PIK3CA-WT (pFastBac)

catalog :

16644

citations: 2

Reference
Miller M, Maheshwari S, Shi W, Gao Y, Chu N, Soares A, et al. Getting the Most Out of Your Crystals: Data Collection at the New High-Flux, Microfocus MX Beamlines at NSLS-II. Molecules. 2019;24: pubmed publisher
Samuels Y, Wang Z, Bardelli A, Silliman N, Ptak J, Szabo S, et al. High frequency of mutations of the PIK3CA gene in human cancers. Science. 2004;304:554 pubmed

product information

Catalog Number :

16644

Product Name :

PIK3CA-WT (pFastBac)

article :

doi	10.1126/science.1096502
id	1874
pubmed_id	15016963

bacterial resistance :

Ampicillin

cloning :

backbone

pFastBac HT

backbone_mutation

backbone_origin

Invitrogen

backbone_size

4856

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Other

Baculoviral

growth notes :

A fragment of human PIK3CA cDNA including the entire ORF was cloned into the pFastBac HT vector. The cDNA fragment can be removed with BamHI and HindIII for subcloning into other vectors.

origin :

pi :

alt_names

Phosphatidylinositol 3-kinase

p110 alpha

cloning

clone_method	Restriction Enzyme
cloning_site_3	HindIII
cloning_site_5	BamHI
promoter
sequencing_primer_3
sequencing_primer_5	polyhedrin forward
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CCM4, CLAPO, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha
gene	PIK3CA
id	5290

genbank_ids

mutation

name

PIK3CA

shRNA_sequence

size

3100

species

9606

Homo sapiens