This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PIK3CA-WT
catalog :
16643
citations: 13
Reference
Gurbuz N, Kahraman N, Sonmez H, Mokhlis H, Koşar P, Ozpolat B. miRNA-193b-5p Suppresses Pancreatic Cancer Cell Proliferation, Invasion, Epithelial Mesenchymal Transition, and Tumor Growth by Inhibiting eEF2K. Anticancer Agents Med Chem. 2022;22:2607-2618 pubmed publisher
Naeem A, Harish V, Coste S, Parasido E, Choudhry M, Kromer L, et al. Regulation of Chemosensitivity in Human Medulloblastoma Cells by p53 and the PI3 Kinase Signaling Pathway. Mol Cancer Res. 2022;20:114-126 pubmed publisher
Zhou Z, Ambrogio C, Bera A, Li Q, Li X, Li L, et al. KRASQ61H preferentially signals through MAPK in a RAF dimer-dependent manner in non-small cell lung cancer. Cancer Res. 2020;: pubmed publisher
Nieuwenhuis B, Barber A, Evans R, Pearson C, Fuchs J, MacQueen A, et al. PI 3-kinase delta enhances axonal PIP3 to support axon regeneration in the adult CNS. EMBO Mol Med. 2020;:e11674 pubmed publisher
Citron F, Segatto I, Rampioni Vinciguerra G, Musco L, Russo F, Mungo G, et al. Downregulation of miR-223 expression is an early event during mammary transformation and confers resistance to CDK4/6 inhibitors in luminal breast cancer. Cancer Res. 2019;: pubmed publisher
Kowshik J, Nivetha R, Ranjani S, Venkatesan P, Selvamuthukumar S, Veeravarmal V, et al. Astaxanthin inhibits hallmarks of cancer by targeting the PI3K/NF-κΒ/STAT3 signalling axis in oral squamous cell carcinoma models. IUBMB Life. 2019;: pubmed publisher
Gao H, Xia Y, Zhang Y, Yang L, Kong L. Vielanin P enhances the cytotoxicity of doxorubicin via the inhibition of PI3K/Nrf2-stimulated MRP1 expression in MCF-7 and K562 DOX-resistant cell lines. Phytomedicine. 2019;58:152885 pubmed publisher
Trautmann M, Cyra M, Isfort I, Jeiler B, Krüger A, Grünewald I, et al. Phosphatidylinositol-3-kinase (PI3K)/Akt Signaling is Functionally Essential in Myxoid Liposarcoma. Mol Cancer Ther. 2019;18:834-844 pubmed publisher
Sophia J, Kowshik J, Dwivedi A, Bhutia S, Manavathi B, Mishra R, et al. Nimbolide, a neem limonoid inhibits cytoprotective autophagy to activate apoptosis via modulation of the PI3K/Akt/GSK-3β signalling pathway in oral cancer. Cell Death Dis. 2018;9:1087 pubmed publisher
Venot Q, Blanc T, Rabia S, Berteloot L, Ladraa S, Duong J, et al. Targeted therapy in patients with PIK3CA-related overgrowth syndrome. Nature. 2018;558:540-546 pubmed publisher
Zhao S, Cao Y, Liu S, Wang X, Bao R, Shu Y, et al. The E545K mutation of PIK3CA promotes gallbladder carcinoma progression through enhanced binding to EGFR. J Exp Clin Cancer Res. 2016;35:97 pubmed publisher
Sathe A, Guerth F, Cronauer M, Heck M, Thalgott M, Gschwend J, et al. Mutant PIK3CA controls DUSP1-dependent ERK 1/2 activity to confer response to AKT target therapy. Br J Cancer. 2014;111:2103-13 pubmed publisher
Samuels Y, Wang Z, Bardelli A, Silliman N, Ptak J, Szabo S, et al. High frequency of mutations of the PIK3CA gene in human cancers. Science. 2004;304:554 pubmed
product information
Catalog Number :
16643
Product Name :
PIK3CA-WT
article :
doi10.1126/science.1096502
id1874
pubmed_id15016963
bacterial resistance :
Kanamycin
cloning :
backbonepCMV2-Tag 2A
backbone_mutation
backbone_originStratagene
backbone_size4300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
This plasmid has 4 mutations in the ORF, N114D, L178S, L401N, and L404P. These mutations do not alter the function of the encoded proteins.
origin :
37
pi :
alt_names
Phosphatidylinositol 3-kinase
p110 alpha
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5EcoRV / XmnI
promoter
sequencing_primer_3T7
sequencing_primer_5T3
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesCCM4, CLAPO, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha
genePIK3CA
id5290
genbank_ids
mutation
namePIK3CA
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagFlag
resistance markers :
553
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA