PIK3CA - Exon9 E545K | Addgene 16642 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

PIK3CA - Exon9 E545K

catalog :

16642

citations: 12

Reference
Huang Y, Wu C, Chang C, Huang S, Kuo H, Shih H. Reciprocal regulation of Daxx and PIK3CA promotes colorectal cancer cell growth. Cell Mol Life Sci. 2022;79:367 pubmed publisher
Pinto N, Ruicci K, Khan M, Shaikh M, Zeng Y, Yoo J, et al. Introduction and expression of PIK3CAE545K in a papillary thyroid cancer BRAFV600E cell line leads to a dedifferentiated aggressive phenotype. J Otolaryngol Head Neck Surg. 2022;51:7 pubmed publisher
Huang S, Chyuan I, Shiue C, Yu M, Hsu Y, Hsu M. Lovastatin-mediated MCF-7 cancer cell death involves LKB1-AMPK-p38MAPK-p53-survivin signalling cascade. J Cell Mol Med. 2020;24:1822-1836 pubmed publisher
Huang S, Yang H, Huang W, Chen W, Yu M, Wang S, et al. WMJ-S-001, a Novel Aliphatic Hydroxamate-Based Compound, Suppresses Lymphangiogenesis Through p38mapk-p53-survivin Signaling Cascade. Front Oncol. 2019;9:1188 pubmed publisher
Venot Q, Blanc T, Rabia S, Berteloot L, Ladraa S, Duong J, et al. Targeted therapy in patients with PIK3CA-related overgrowth syndrome. Nature. 2018;558:540-546 pubmed publisher
Li J, Liu C, Shiao W, Jayakumar T, Li Y, Chang N, et al. Inhibitory effect of PDGF-BB and serum-stimulated responses in vascular smooth muscle cell proliferation by hinokitiol via up-regulation of p21 and p53. Arch Med Sci. 2018;14:579-587 pubmed publisher
Kidacki M, Lehman H, Green M, Warrick J, Stairs D. p120-Catenin Downregulation and PIK3CA Mutations Cooperate to Induce Invasion through MMP1 in HNSCC. Mol Cancer Res. 2017;15:1398-1409 pubmed publisher
Zhao S, Cao Y, Liu S, Wang X, Bao R, Shu Y, et al. The E545K mutation of PIK3CA promotes gallbladder carcinoma progression through enhanced binding to EGFR. J Exp Clin Cancer Res. 2016;35:97 pubmed publisher
Huang Y, Huang S, Hsu Y, Ou G, Huang W, Hsu M. The effects of a novel aliphatic-chain hydroxamate derivative WMJ-S-001 in HCT116 colorectal cancer cell death. Sci Rep. 2015;5:15900 pubmed publisher
Pathak R, Shao L, Chafekar S, Feng W, Ponnappan U, Fink L, et al. IKK? regulates endothelial thrombomodulin in a Klf2-dependent manner. J Thromb Haemost. 2014;12:1533-1544 pubmed publisher
Chang H, Chen C, Hsu Y, Kuo W, Ou G, Chiu P, et al. Simvastatin induced HCT116 colorectal cancer cell apoptosis through p38MAPK-p53-survivin signaling cascade. Biochim Biophys Acta. 2013;1830:4053-64 pubmed publisher
Samuels Y, Wang Z, Bardelli A, Silliman N, Ptak J, Szabo S, et al. High frequency of mutations of the PIK3CA gene in human cancers. Science. 2004;304:554 pubmed

product information

Catalog Number :

16642

Product Name :

PIK3CA - Exon9 E545K

article :

doi	10.1126/science.1096502
id	1874
pubmed_id	15016963

bacterial resistance :

Kanamycin

cloning :

backbone

pCMV2-Tag 2A

backbone_mutation

backbone_origin

Stratagene

backbone_size

4300

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

This plasmid has several discrepancies in the ORF compared to the NCBI reference (NM_006218.3): N114D, L177S, T322A, A400V, L404P. These mutations do not alter the function of the encoded protein as described in the associated publication.

origin :

pi :

alt_names

Phosphatidylinositol 3-kinase

p110 alpha

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	EcoRV / XmnI
promoter
sequencing_primer_3	T7
sequencing_primer_5	T3
site_3_destroyed
site_5_destroyed	1

entrez_gene

aliases	CCM4, CLAPO, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha
gene	PIK3CA
id	5290

genbank_ids

mutation

E545K; see note below

name

PIK3CA E545K

shRNA_sequence

size

species

9606

Homo sapiens