This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PIK3CA - Exon20 H1047R (pFastBac)
catalog :
16641
citations: 1
Reference
Samuels Y, Wang Z, Bardelli A, Silliman N, Ptak J, Szabo S, et al. High frequency of mutations of the PIK3CA gene in human cancers. Science. 2004;304:554 pubmed
product information
Catalog Number :
16641
Product Name :
PIK3CA - Exon20 H1047R (pFastBac)
article :
doi10.1126/science.1096502
id1874
pubmed_id15016963
bacterial resistance :
Ampicillin
cloning :
backbonepFastBac HT
backbone_mutation
backbone_originInvitrogen
backbone_size4856
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
Baculoviral
growth notes :
A fragment of human PIK3CA cDNA including the entire ORF was cloned into the pFastBac HT vector. The cDNA fragment can be removed with BamHI and HindIII for subcloning into other vectors.
origin :
37
pi :
alt_names
Phosphatidylinositol 3-kinase
p110 alpha
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5polyhedrin forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCCM4, CLAPO, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha
genePIK3CA
id5290
genbank_ids
mutationH1047R
namePIK3CA H1047R
shRNA_sequence
size3100
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHis
resistance markers :
553
tags :
Unknown
terms :
Gentamicin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA