This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PIK3CA - Exon20 H1047R
catalog :
16639
citations: 6
Reference
Huang Y, Wu C, Chang C, Huang S, Kuo H, Shih H. Reciprocal regulation of Daxx and PIK3CA promotes colorectal cancer cell growth. Cell Mol Life Sci. 2022;79:367 pubmed publisher
Citron F, Segatto I, Rampioni Vinciguerra G, Musco L, Russo F, Mungo G, et al. Downregulation of miR-223 expression is an early event during mammary transformation and confers resistance to CDK4/6 inhibitors in luminal breast cancer. Cancer Res. 2019;: pubmed publisher
Trautmann M, Cyra M, Isfort I, Jeiler B, Krüger A, Grünewald I, et al. Phosphatidylinositol-3-kinase (PI3K)/Akt Signaling is Functionally Essential in Myxoid Liposarcoma. Mol Cancer Ther. 2019;18:834-844 pubmed publisher
Venot Q, Blanc T, Rabia S, Berteloot L, Ladraa S, Duong J, et al. Targeted therapy in patients with PIK3CA-related overgrowth syndrome. Nature. 2018;558:540-546 pubmed publisher
Kidacki M, Lehman H, Green M, Warrick J, Stairs D. p120-Catenin Downregulation and PIK3CA Mutations Cooperate to Induce Invasion through MMP1 in HNSCC. Mol Cancer Res. 2017;15:1398-1409 pubmed publisher
Samuels Y, Wang Z, Bardelli A, Silliman N, Ptak J, Szabo S, et al. High frequency of mutations of the PIK3CA gene in human cancers. Science. 2004;304:554 pubmed
product information
Catalog Number :
16639
Product Name :
PIK3CA - Exon20 H1047R
article :
doi10.1126/science.1096502
id1874
pubmed_id15016963
bacterial resistance :
Kanamycin
cloning :
backbonepCMV2-Tag 2A
backbone_mutation
backbone_originStratagene
backbone_size4300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
This plasmid has 2 additional mutations in the ORF, N115D and L178S. These mutations do not alter the function of the encoded proteins.
origin :
37
pi :
alt_names
Phosphatidylinositol 3-kinase
p110 alpha
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5EcoRV / XmnI
promoter
sequencing_primer_3T7
sequencing_primer_5T3
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesCCM4, CLAPO, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha
genePIK3CA
id5290
genbank_ids
mutationH1047R
namePIK3CA H1047R
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagFlag
resistance markers :
553
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA