pCI-Neo-PTPRT (R632X) | Addgene 16629 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCI-Neo-PTPRT (R632X)

catalog :

16629

citations: 1

Reference
Wang Z, Shen D, Parsons D, Bardelli A, Sager J, Szabo S, et al. Mutational analysis of the tyrosine phosphatome in colorectal cancers. Science. 2004;304:1164-6 pubmed

product information

Catalog Number :

16629

Product Name :

pCI-Neo-PTPRT (R632X)

article :

doi	10.1126/science.1096096
id	1873
pubmed_id	15155950

bacterial resistance :

Ampicillin

cloning :

backbone

pCI-Neo

backbone_mutation

backbone_origin

Promega

backbone_size

5472

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

A 6kb fragment of PTPRT including the entire ORF and the 3'UTR was excised from KIAA clone (ha06139s1) with SgrAI and ScaI and cloned into the XmaI/EcoRV sites of pIRES2hyg2. A 1.9kb fragment containing PTPRT was digested with NheI and NotI and cloned into the pCI-Neo expression vector.

origin :

pi :

alt_names

protein tyrosine phosphatase, receptor type, T

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	NheI
promoter
sequencing_primer_3	EBV rev
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	RPTPrho
gene	PTPRT
id	11122

genbank_ids

mutation

truncated R632X mutant

name

PTPRT R632X

shRNA_sequence

size

1900

species

9606

Homo sapiens