pTBRII / CEP-Zeo / Hygro | Addgene 16622 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pTBRII / CEP-Zeo / Hygro

catalog :

16622

citations: 1

Reference
Zhou S, Buckhaults P, Zawel L, Bunz F, Riggins G, Dai J, et al. Targeted deletion of Smad4 shows it is required for transforming growth factor beta and activin signaling in colorectal cancer cells. Proc Natl Acad Sci U S A. 1998;95:2412-6 pubmed

product information

Catalog Number :

16622

Product Name :

pTBRII / CEP-Zeo / Hygro

article :

doi	10.1073/pnas.95.5.2412
id	1871
pubmed_id	9482899

bacterial resistance :

Ampicillin

cloning :

backbone

pCEP4

backbone_mutation

pCMV-Zeo cassette inserted into pCEP4

backbone_origin

Invitrogen

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

An expression construct for the TGF-beta RII gene was constructed by placing a TGF-beta RII cDNA between a cytomegalovirus (CMV) promoter and an simian virus 40 polyadenylation signal. Please note that according to the map, this plasmid has 2 CMV promoters, so sequence confirmation with this primer may have limited success. Restriction digest with BamHI/HindIII is recommended for plasmid confirmation.

origin :

pi :

alt_names

TGFbRII

transforming growth factor, beta receptor II

cloning

clone_method	Restriction Enzyme
cloning_site_3	HindIII
cloning_site_5	BamHI
promoter
sequencing_primer_3
sequencing_primer_5	pGEX3
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AAT3, FAA3, LDS1B, LDS2, LDS2B, MFS2, RIIC, TAAD2, TBR-ii, TBRII, TGFR-2, TGFbeta-RII, tbetaR-II
gene	TGFBR2
id	7048

genbank_ids

mutation

name

TGF beta type II receptor

shRNA_sequence

size

species

9606

Homo sapiens