This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pNSU299_RPL22A
catalog :
166079
product information
Catalog Number :
166079
Product Name :
pNSU299_RPL22A
article :
doi
id28215970
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepRS414
backbone_mutation
backbone_originSmith JD et al 2016. PMID 26956608
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
CRISPR
growth notes :
gRNA Binding Coordinates: chrXII:263006. 263025 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome. Addgene QC identified discrepancies compared to the theoretical sequence. The depositing lab confirms the functional activity of the construct despite these differences.
growth strain :
Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL22A for double stranded break formation in yeast.
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterTet-inducible
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYLR061W
geneRPL22A
id850750
genbank_ids
mutation
namePromoter of RPL22A
shRNA_sequenceTTCAAAAGAGAAGCGCACTG
size
species
4932
Saccharomyces cerevisiae
32644
Other
tags
plasmid copy :
Jim Haber/Neal Sugawara
resistance markers :
5337
tags :
High Copy
terms :
TRP1
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA