c-myc promoter (TBE 1/2 - wt) | Addgene 16595 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

c-myc promoter (TBE 1/2 - wt)

catalog :

16595

citations: 3

Reference
Ripple M, Parker Struckhoff A, Trillo Tinoco J, Li L, Margolin D, McGoey R, et al. Activation of c-Myc and Cyclin D1 by JCV T-Antigen and Î²-catenin in colon cancer. PLoS ONE. 2014;9:e106257 pubmed publisher
Luo H, Rankin G, Juliano N, Jiang B, Chen Y. Kaempferol inhibits VEGF expression and in vitro angiogenesis through a novel ERK-NFÎºB-cMyc-p21 pathway. Food Chem. 2012;130:321-328 pubmed
He T, Sparks A, Rago C, Hermeking H, Zawel L, da Costa L, et al. Identification of c-MYC as a target of the APC pathway. Science. 1998;281:1509-12 pubmed

product information

Catalog Number :

16595

Product Name :

c-myc promoter (TBE 1/2 - wt)

article :

doi	10.1126/science.281.5382.1509
id	1865
pubmed_id	9727977

bacterial resistance :

Ampicillin

cloning :

backbone

pBV-Luc

backbone_mutation

backbone_origin

Vogelstein Lab

backbone_size

4867

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Luciferase

growth notes :

To construct pTBE1/2 plasmid, we used polymerase chain reaction (PCR) primers (5'-CTAGCTAGCCTAGCACCTTTGATTTCTCCC-3' and 5'-CGTGATATCCGCTTTGATCAAGAGTCCCAG-3') to amplify nt -576 to -1162 of the c-MYC promoter region. The PCR product was cloned into pBV-Luc. TBE1 (Tcf-4 binding elements) wt sequence cctttgatt; TBE2 wt sequence gctttgatc. See Author's Map and article for more information.

origin :

pi :

alt_names

MYC

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRV
cloning_site_5	NheI
promoter
sequencing_primer_3	LucNRev
sequencing_primer_5	n/a
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MRTL, MYCC, bHLHe39, c-Myc
gene	MYC
id	4609

genbank_ids

mutation

name

c-myc promoter (TBE 1/2 wt)

shRNA_sequence

size

600

species

9606

Homo sapiens