This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
4XBS2WT-Luc
catalog :
16593
citations: 3
Reference
Gudikote J, Cascone T, Poteete A, Sitthideatphaiboon P, Wu Q, Morikawa N, et al. Inhibition of nonsense-mediated decay rescues p53β/γ isoform expression and activates the p53 pathway in MDM2-overexpressing and select p53-mutant cancers. J Biol Chem. 2021;297:101163 pubmed publisher
Hennigan R, Fletcher J, Guard S, Ratner N. Proximity biotinylation identifies a set of conformation-specific interactions between Merlin and cell junction proteins. Sci Signal. 2019;12: pubmed publisher
Yu J, Zhang L, Hwang P, Kinzler K, Vogelstein B. PUMA induces the rapid apoptosis of colorectal cancer cells. Mol Cell. 2001;7:673-82 pubmed
product information
Catalog Number :
16593
Product Name :
4XBS2WT-Luc
article :
doi10.1016/S1097-2765(01)00213-1
id1864
pubmed_id11463391
bacterial resistance :
Ampicillin
cloning :
backbonepBV-Luc
backbone_mutation
backbone_originVogelstein Lab
backbone_size4867
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Luciferase
growth notes :
The following oligo pairs containing two copies of wild-type p53 binding sites were used: 5'-CTAGGCTGCA AGTCCTGACT TGTCCACACT CTGCAAGTCC TGACTTGTCC-3' and 5'-CTAGGGACAA GTCAGGACTT GCAGAGTGTG GACAAGTCAG GACTTGCAGC-3'. The annealed oligonucleotide pairs were concatamerized and cloned into the NheI site of pBVLuc.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NheI
cloning_site_5NheI
promoter
sequencing_primer_3LucNrev
sequencing_primer_5RVprimer3
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesBCC7, BMFS5, LFS1, P53, TRP53
geneTP53
id7157
genbank_ids
mutation
namep53 binding sites
shRNA_sequence
size100
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagLuciferase
resistance markers :
553
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA