This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSin-EF2-Oct4-Pur
catalog :
16579
citations: 23
Reference
Cheng Y, Shen Z, Gao Y, Chen F, Xu H, Mo Q, et al. Phase transition and remodeling complex assembly are important for SS18-SSX oncogenic activity in synovial sarcomas. Nat Commun. 2022;13:2724 pubmed publisher
Filipponi D, Pagnuzzi Boncompagni M, Pages G. Inhibiting ALK2/ALK3 Signaling to Differentiate and Chemo-Sensitize Medulloblastoma. Cancers (Basel). 2022;14: pubmed publisher
Deshpande R, Sharma S, Liu Y, Pandey P, Pei X, Wu K, et al. LncRNA IPW inhibits growth of ductal carcinoma in situ by downregulating ID2 through miR-29c. Breast Cancer Res. 2022;24:6 pubmed publisher
Morgan M, Popova I, Vaidya A, Burg J, Marunde M, Rendleman E, et al. A trivalent nucleosome interaction by PHIP/BRWD2 is disrupted in neurodevelopmental disorders and cancer. Genes Dev. 2021;35:1642-1656 pubmed publisher
Generali M, Satheesha S, Bode P, Wanner D, Schafer B, Casanova E. High Frequency of Tumor Propagating Cells in Fusion-Positive Rhabdomyosarcoma. Genes (Basel). 2021;12: pubmed publisher
Lu C, Shiau A, Su B, Hsu T, Wang C, Su Y, et al. Oct4 promotes M2 macrophage polarization through upregulation of macrophage colony-stimulating factor in lung cancer. J Hematol Oncol. 2020;13:62 pubmed publisher
Endo M, Druso J, Cerione R. The two splice variant forms of Cdc42 exert distinct and essential functions in neurogenesis. J Biol Chem. 2020;295:4498-4512 pubmed publisher
Ruiz G, Valencia González H, Perez Montiel D, Muñoz F, Ocadiz Delgado R, Fernandez Retana J, et al. Genes Involved in the Transcriptional Regulation of Pluripotency Are Expressed in Malignant Tumors of the Uterine Cervix and Can Induce Tumorigenic Capacity in a Nontumorigenic Cell Line. Stem Cells Int. 2019;2019:7683817 pubmed publisher
Peskova L, Cerná K, Oppelt J, Mraz M, Bárta T. Oct4-mediated reprogramming induces embryonic-like microRNA expression signatures in human fibroblasts. Sci Rep. 2019;9:15759 pubmed publisher
Durán Alonso M, López Hernández I, de la Fuente M, Garcia Sancho J, Giraldez F, Schimmang T. Transcription factor induced conversion of human fibroblasts towards the hair cell lineage. PLoS ONE. 2018;13:e0200210 pubmed publisher
Bak M, FURMANSKI P, Shan N, Lee H, Bao C, Lin Y, et al. Tocopherols inhibit estrogen-induced cancer stemness and OCT4 signaling in breast cancer. Carcinogenesis. 2018;: pubmed publisher
Zeng S, Liu L, Ouyang Q, Zhao Y, Lin G, Hu L, et al. Generation of induced pluripotent stem cells (iPSCs) from a retinoblastoma patient carrying a c.2663G>A mutation in RB1 gene. Stem Cell Res. 2016;17:208-211 pubmed publisher
Chang W, Hwu Y, Xu J, Lin C, Wang S, Cheng A, et al. Derivation of Patient Specific Pluripotent Stem Cells Using Clinically Discarded Cumulus Cells. PLoS ONE. 2016;11:e0165715 pubmed publisher
Lu C, Shieh G, Wang C, Su B, Su Y, Chen Y, et al. Chemotherapeutics-induced Oct4 expression contributes to drug resistance and tumor recurrence in bladder cancer. Oncotarget. 2017;8:30844-30858 pubmed publisher
Liu Z, Skamagki M, Kim K, Zhao R. Canonical MicroRNA Activity Facilitates but May Be Dispensable for Transcription Factor-Mediated Reprogramming. Stem Cell Reports. 2015;5:1119-1127 pubmed publisher
Leng L, Tan Y, Gong F, Hu L, Ouyang Q, Zhao Y, et al. Differentiation of primordial germ cells from induced pluripotent stem cells of primary ovarian insufficiency. Hum Reprod. 2015;30:737-48 pubmed publisher
Tahmasebi S, Ghorbani M, Savage P, Gocevski G, Yang X. The SUMO conjugating enzyme Ubc9 is required for inducing and maintaining stem cell pluripotency. Stem Cells. 2014;32:1012-20 pubmed publisher
Mitchell R, Szabo E, Benoit Y, Case D, Mechael R, Alamilla J, et al. Activation of neural cell fate programs toward direct conversion of adult human fibroblasts into tri-potent neural progenitors using OCT-4. Stem Cells Dev. 2014;23:1937-46 pubmed publisher
Zhang L, Pan Y, Qin G, Chen L, Chatterjee T, Weintraub N, et al. Inhibition of stearoyl-coA desaturase selectively eliminates tumorigenic Nanog-positive cells: improving the safety of iPS cell transplantation to myocardium. Cell Cycle. 2014;13:762-71 pubmed publisher
Tahmasebi S, Ghorbani M, Savage P, Yan K, Gocevski G, Xiao L, et al. Sumoylation of Krüppel-like factor 4 inhibits pluripotency induction but promotes adipocyte differentiation. J Biol Chem. 2013;288:12791-804 pubmed publisher
Kumar S, Liu S, Lu H, Zhang H, Zhang P, Gimotty P, et al. Acquired cancer stem cell phenotypes through Oct4-mediated dedifferentiation. Oncogene. 2012;31:4898-911 pubmed publisher
Ruan J, Shen J, Wang Z, Ji J, Song H, Wang K, et al. Efficient preparation and labeling of human induced pluripotent stem cells by nanotechnology. Int J Nanomedicine. 2011;6:425-35 pubmed publisher
Yu J, Vodyanik M, Smuga Otto K, Antosiewicz Bourget J, Frane J, Tian S, et al. Induced pluripotent stem cell lines derived from human somatic cells. Science. 2007;318:1917-20 pubmed
product information
Catalog Number :
16579
Product Name :
pSin-EF2-Oct4-Pur
article :
doi10.1126/science.1151526
id1807
pubmed_id18029452
bacterial resistance :
Ampicillin
cloning :
backbonepSin4-EF2-IRES-Pur
backbone_mutation
backbone_origin
backbone_size7500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
The psPAX2 packaging plasmid and pMD2.G envelope plasmid can be used with this vector.
origin :
37
pi :
alt_names
POU5F1
Oct4
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5SpeI
promoter
sequencing_primer_3n/a
sequencing_primer_5EF-1a Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesOCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4
genePOU5F1
id5460
genbank_ids
NM_002701
mutation
nameHomo sapiens POU domain, class 5, transcription factor 1 (POU5F1)
shRNA_sequence
size1100
species
9606
Homo sapiens
tags
plasmid copy :
SINF-EF-G (from Dr. Robert Hawley, George Washington University) was modified to make the lentiviral backbone.
resistance markers :
523
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA