This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSin-EF2-Nanog-Pur
catalog :
16578
citations: 27
Reference
Tsai L, Peng M, Chang C, Wen L, Liu L, Liang X, et al. ZSCAN4 interacts with PARP1 to promote DNA repair in mouse embryonic stem cells. Cell Biosci. 2023;13:193 pubmed publisher
Oh S, Lim J, Son M, Ahn J, Song K, Lee H, et al. TRPV1 inhibition overcomes cisplatin resistance by blocking autophagy-mediated hyperactivation of EGFR signaling pathway. Nat Commun. 2023;14:2691 pubmed publisher
Díaz Tejeda Y, Guido Jimenez M, López Carbajal H, Amador Molina A, Méndez Martínez R, Gariglio Vidal P, et al. Nanog, in Cooperation with AP1, Increases the Expression of E6/E7 Oncogenes from HPV Types 16/18. Viruses. 2021;13: pubmed publisher
Chen K, Oztürk K, Liefeld T, Reich M, Mesirov J, Carter H, et al. A phenotypically supervised single-cell analysis protocol to study within-cell-type heterogeneity of cultured mammalian cells. STAR Protoc. 2021;2:100561 pubmed publisher
Chang W, Peng M, Hsu J, Xu J, Cho H, Hsieh Li H, et al. Effects of Survival Motor Neuron Protein on Germ Cell Development in Mouse and Human. Int J Mol Sci. 2021;22: pubmed publisher
Ruiz G, Valencia González H, Perez Montiel D, Muñoz F, Ocadiz Delgado R, Fernandez Retana J, et al. Genes Involved in the Transcriptional Regulation of Pluripotency Are Expressed in Malignant Tumors of the Uterine Cervix and Can Induce Tumorigenic Capacity in a Nontumorigenic Cell Line. Stem Cells Int. 2019;2019:7683817 pubmed publisher
Uhlmann C, Kuhn L, Tigges J, Fritsche E, Kahlert U. Efficient Modulation of TP53 Expression in Human Induced Pluripotent Stem Cells. Curr Protoc Stem Cell Biol. 2020;52:e102 pubmed publisher
García Olloqui P, Rodríguez Madoz J, Di Scala M, Abizanda G, Vales A, Olague C, et al. Effect of heart ischemia and administration route on biodistribution and transduction efficiency of AAV9 vectors. J Tissue Eng Regen Med. 2020;14:123-134 pubmed publisher
Wang R, Pscheid R, Ghumra A, Kan L, Cochrane S, Fairclough L, et al. Towards a surrogate system to express human lipid binding TCRs. Biotechnol Lett. 2019;41:1095-1104 pubmed publisher
Wang X, Sun D, Tai J, Chen S, Yu M, Ren D, et al. TFAP2C promotes stemness and chemotherapeutic resistance in colorectal cancer via inactivating hippo signaling pathway. J Exp Clin Cancer Res. 2018;37:27 pubmed publisher
Lim S, Macheleidt I, Dalvi P, Schäfer S, Kerick M, Ozretić L, et al. LSD1 modulates the non-canonical integrin β3 signaling pathway in non-small cell lung carcinoma cells. Sci Rep. 2017;7:10292 pubmed publisher
Talbot N, Sparks W, Phillips C, Ealy A, Powell A, Caperna T, et al. Bovine trophectoderm cells induced from bovine fibroblasts with induced pluripotent stem cell reprogramming factors. Mol Reprod Dev. 2017;84:468-485 pubmed publisher
Shah M, Cardenas R, Wang B, Persson J, Mongan N, Grabowska A, et al. HOXC8 regulates self-renewal, differentiation and transformation of breast cancer stem cells. Mol Cancer. 2017;16:38 pubmed publisher
Zhang X, Ren D, Guo L, Wang L, Wu S, Lin C, et al. Thymosin beta 10 is a key regulator of tumorigenesis and metastasis and a novel serum marker in breast cancer. Breast Cancer Res. 2017;19:15 pubmed publisher
Tsanov K, Pearson D, Wu Z, Han A, Triboulet R, Seligson M, et al. LIN28 phosphorylation by MAPK/ERK couples signalling to the post-transcriptional control of pluripotency. Nat Cell Biol. 2017;19:60-67 pubmed publisher
Zeng S, Liu L, Ouyang Q, Zhao Y, Lin G, Hu L, et al. Generation of induced pluripotent stem cells (iPSCs) from a retinoblastoma patient carrying a c.2663G>A mutation in RB1 gene. Stem Cell Res. 2016;17:208-211 pubmed publisher
Chang W, Hwu Y, Xu J, Lin C, Wang S, Cheng A, et al. Derivation of Patient Specific Pluripotent Stem Cells Using Clinically Discarded Cumulus Cells. PLoS ONE. 2016;11:e0165715 pubmed publisher
Hanna J, Garcia M, Go J, Finkelstein D, Kodali K, Pagala V, et al. PAX7 is a required target for microRNA-206-induced differentiation of fusion-negative rhabdomyosarcoma. Cell Death Dis. 2016;7:e2256 pubmed publisher
Tenga A, Beard J, Takwi A, Wang Y, Chen T. Regulation of Nuclear Receptor Nur77 by miR-124. PLoS ONE. 2016;11:e0148433 pubmed publisher
Palla A, Piazzolla D, Alcazar N, Cañamero M, Graña O, Gómez López G, et al. The pluripotency factor NANOG promotes the formation of squamous cell carcinomas. Sci Rep. 2015;5:10205 pubmed publisher
Gayle S, Pan Y, Landrette S, Xu T. piggyBac insertional mutagenesis screen identifies a role for nuclear RHOA in human ES cell differentiation. Stem Cell Reports. 2015;4:926-38 pubmed publisher
Leng L, Tan Y, Gong F, Hu L, Ouyang Q, Zhao Y, et al. Differentiation of primordial germ cells from induced pluripotent stem cells of primary ovarian insufficiency. Hum Reprod. 2015;30:737-48 pubmed publisher
Langlois T, da Costa Reis Monte Mor B, Lenglet G, Droin N, Marty C, Le Couedic J, et al. TET2 deficiency inhibits mesoderm and hematopoietic differentiation in human embryonic stem cells. Stem Cells. 2014;32:2084-97 pubmed publisher
Liao W, Liaw C, Huang Y, Han H, Hsu H, Hwang S, et al. Cyclohexylmethyl Flavonoids Suppress Propagation of Breast Cancer Stem Cells via Downregulation of NANOG. Evid Based Complement Alternat Med. 2013;2013:170261 pubmed publisher
Wang X, Wang J, Huang V, Place R, Li L. Induction of NANOG expression by targeting promoter sequence with small activating RNA antagonizes retinoic acid-induced differentiation. Biochem J. 2012;443:821-8 pubmed publisher
Dick E, Matsa E, Young L, Darling D, Denning C. Faster generation of hiPSCs by coupling high-titer lentivirus and column-based positive selection. Nat Protoc. 2011;6:701-14 pubmed publisher
Yu J, Vodyanik M, Smuga Otto K, Antosiewicz Bourget J, Frane J, Tian S, et al. Induced pluripotent stem cell lines derived from human somatic cells. Science. 2007;318:1917-20 pubmed
product information
Catalog Number :
16578
Product Name :
pSin-EF2-Nanog-Pur
article :
doi10.1126/science.1151526
id1807
pubmed_id18029452
bacterial resistance :
Ampicillin
cloning :
backbonepSin4-EF2-IRES-Pur
backbone_mutation
backbone_origin
backbone_size7500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
The psPAX2 packaging plasmid and pMD2.G envelope plasmid can be used with this vector.
origin :
37
pi :
alt_names
NANOG
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5SpeI
promoter
sequencing_primer_3n/a
sequencing_primer_5EF-1a Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneNANOG
id79923
genbank_ids
NM_024865
mutation
nameHomo sapiens Nanog homeobox
shRNA_sequence
size900
species
9606
Homo sapiens
tags
plasmid copy :
SINF-EF-G (from Dr. Robert Hawley, George Washington University) was modified to make the lentiviral backbone.
resistance markers :
523
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA