This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSin-EF2-Sox2-Pur
catalog :
16577
citations: 26
Reference
Xie Q, Hua X, Huang C, Liao X, Tian Z, Xu J, et al. SOX2 Promotes Invasion in Human Bladder Cancers through MMP2 Upregulation and FOXO1 Downregulation. Int J Mol Sci. 2022;23: pubmed publisher
Wu T, Rabi S, Michaud W, Becerra D, Gilpin S, Mino Kenudson M, et al. Protease inhibitor Camostat Mesyalte blocks wild type SARS-CoV-2 and D614G viral entry in human engineered miniature lungs. Biomaterials. 2022;285:121509 pubmed publisher
Generali M, Satheesha S, Bode P, Wanner D, Schafer B, Casanova E. High Frequency of Tumor Propagating Cells in Fusion-Positive Rhabdomyosarcoma. Genes (Basel). 2021;12: pubmed publisher
Li J, Haque M, Shang C, Hassan B, Liu D, Chen W, et al. Identification and Characterization of Cancer Stem-Like Cells in ALK-Positive Anaplastic Large Cell Lymphoma Using the SORE6 Reporter. Curr Issues Mol Biol. 2021;43:543-557 pubmed publisher
Gray K, Bordt E, Atyeo C, Deriso E, Akinwunmi B, Young N, et al. Coronavirus disease 2019 vaccine response in pregnant and lactating women: a cohort study. Am J Obstet Gynecol. 2021;: pubmed publisher
Wang S, Liu J, Qin J, Zhu Y, Tin V, Yam J, et al. CAMK2A supported tumor initiating cells of lung adenocarcinoma by upregulating SOX2 through EZH2 phosphorylation. Cell Death Dis. 2020;11:410 pubmed publisher
Wu H, Du C, Yang F, Zheng X, Qiu D, Zhang Q, et al. Generation of hepatocyte-like cells from human urinary epithelial cells and the role of autophagy during direct reprogramming. Biochem Biophys Res Commun. 2020;527:723-729 pubmed publisher
Menendez S, Rey V, Martinez Cruzado L, Gonzalez M, Morales Molina Á, Santos L, et al. SOX2 Expression and Transcriptional Activity Identifies a Subpopulation of Cancer Stem Cells in Sarcoma with Prognostic Implications. Cancers (Basel). 2020;12: pubmed publisher
Ruiz G, Valencia González H, Perez Montiel D, Muñoz F, Ocadiz Delgado R, Fernandez Retana J, et al. Genes Involved in the Transcriptional Regulation of Pluripotency Are Expressed in Malignant Tumors of the Uterine Cervix and Can Induce Tumorigenic Capacity in a Nontumorigenic Cell Line. Stem Cells Int. 2019;2019:7683817 pubmed publisher
Arévalo Martínez M, Cidad P, García Mateo N, Moreno Estar S, Serna J, Fernández M, et al. Myocardin-Dependent Kv1.5 Channel Expression Prevents Phenotypic Modulation of Human Vessels in Organ Culture. Arterioscler Thromb Vasc Biol. 2019;39:e273-e286 pubmed publisher
Liu X, Qiao B, Zhao T, Hu F, Lam A, Tao Q. Sox2 promotes tumor aggressiveness and epithelial‑mesenchymal transition in tongue squamous cell carcinoma. Int J Mol Med. 2018;42:1418-1426 pubmed publisher
Hüser L, Sachindra S, Granados K, Federico A, Larribere L, Novak D, et al. SOX2-mediated upregulation of CD24 promotes adaptive resistance toward targeted therapy in melanoma. Int J Cancer. 2018;143:3131-3142 pubmed publisher
Li X, Michaeloudes C, Zhang Y, Wiegman C, Adcock I, Lian Q, et al. Mesenchymal stem cells alleviate oxidative stress-induced mitochondrial dysfunction in the airways. J Allergy Clin Immunol. 2018;141:1634-1645.e5 pubmed publisher
Javidi Parsijani P, Niu G, Davis M, Lu P, Atala A, Lu B. No evidence of genome editing activity from Natronobacterium gregoryi Argonaute (NgAgo) in human cells. PLoS ONE. 2017;12:e0177444 pubmed publisher
Zeng S, Liu L, Ouyang Q, Zhao Y, Lin G, Hu L, et al. Generation of induced pluripotent stem cells (iPSCs) from a retinoblastoma patient carrying a c.2663G>A mutation in RB1 gene. Stem Cell Res. 2016;17:208-211 pubmed publisher
Chang W, Hwu Y, Xu J, Lin C, Wang S, Cheng A, et al. Derivation of Patient Specific Pluripotent Stem Cells Using Clinically Discarded Cumulus Cells. PLoS ONE. 2016;11:e0165715 pubmed publisher
Boehm E, Zornoza M, Jourdain A, Delmiro Magdalena A, Garcia Consuegra I, Torres Merino R, et al. Role of FAST Kinase Domains 3 (FASTKD3) in Post-transcriptional Regulation of Mitochondrial Gene Expression. J Biol Chem. 2016;291:25877-25887 pubmed
Li X, Zhang Y, Liang Y, Cui Y, Yeung S, Ip M, et al. iPSC-derived mesenchymal stem cells exert SCF-dependent recovery of cigarette smoke-induced apoptosis/proliferation imbalance in airway cells. J Cell Mol Med. 2017;21:265-277 pubmed publisher
Leng L, Tan Y, Gong F, Hu L, Ouyang Q, Zhao Y, et al. Differentiation of primordial germ cells from induced pluripotent stem cells of primary ovarian insufficiency. Hum Reprod. 2015;30:737-48 pubmed publisher
Tahmasebi S, Ghorbani M, Savage P, Gocevski G, Yang X. The SUMO conjugating enzyme Ubc9 is required for inducing and maintaining stem cell pluripotency. Stem Cells. 2014;32:1012-20 pubmed publisher
Santini R, Pietrobono S, Pandolfi S, Montagnani V, D Amico M, Penachioni J, et al. SOX2 regulates self-renewal and tumorigenicity of human melanoma-initiating cells. Oncogene. 2014;33:4697-708 pubmed publisher
Zhang L, Pan Y, Qin G, Chen L, Chatterjee T, Weintraub N, et al. Inhibition of stearoyl-coA desaturase selectively eliminates tumorigenic Nanog-positive cells: improving the safety of iPS cell transplantation to myocardium. Cell Cycle. 2014;13:762-71 pubmed publisher
Rybak A, Tang D. SOX2 plays a critical role in EGFR-mediated self-renewal of human prostate cancer stem-like cells. Cell Signal. 2013;25:2734-42 pubmed publisher
Tahmasebi S, Ghorbani M, Savage P, Yan K, Gocevski G, Xiao L, et al. Sumoylation of Krüppel-like factor 4 inhibits pluripotency induction but promotes adipocyte differentiation. J Biol Chem. 2013;288:12791-804 pubmed publisher
Ruan J, Shen J, Wang Z, Ji J, Song H, Wang K, et al. Efficient preparation and labeling of human induced pluripotent stem cells by nanotechnology. Int J Nanomedicine. 2011;6:425-35 pubmed publisher
Yu J, Vodyanik M, Smuga Otto K, Antosiewicz Bourget J, Frane J, Tian S, et al. Induced pluripotent stem cell lines derived from human somatic cells. Science. 2007;318:1917-20 pubmed
product information
Catalog Number :
16577
Product Name :
pSin-EF2-Sox2-Pur
article :
doi10.1126/science.1151526
id1807
pubmed_id18029452
bacterial resistance :
Ampicillin
cloning :
backbonepSin4-EF2-IRES-Pur
backbone_mutation
backbone_origin
backbone_size7500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
The psPAX2 packaging plasmid and pMD2.G envelope plasmid can be used with this vector.
origin :
37
pi :
alt_names
Sox2
cloning
clone_methodRestriction Enzyme
cloning_site_3SpeI
cloning_site_5EcoRI
promoter
sequencing_primer_3n/a
sequencing_primer_5EF-1a Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesANOP3, MCOPS3
geneSOX2
id6657
genbank_ids
NM_003106
mutation
nameHomo sapiens SRY (sex determining region Y)-box 2 (SOX2)
shRNA_sequence
size1000
species
9606
Homo sapiens
tags
plasmid copy :
SINF-EF-G (from Dr. Robert Hawley, George Washington University) was modified to make the lentiviral backbone.
resistance markers :
523
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA