pBV-Luc mut MBS4 | Addgene 16570 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pBV-Luc mut MBS4

catalog :

16570

citations: 1

Reference
Hermeking H, Rago C, Schuhmacher M, Li Q, Barrett J, Obaya A, et al. Identification of CDK4 as a target of c-MYC. Proc Natl Acad Sci U S A. 2000;97:2229-34 pubmed

product information

Catalog Number :

16570

Product Name :

pBV-Luc mut MBS4

article :

doi	10.1073/pnas.050586197
id	1858
pubmed_id	10688915

bacterial resistance :

Ampicillin

cloning :

backbone

pBV-Luc

backbone_mutation

backbone_origin

Vogelstein Lab

backbone_size

4867

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Luciferase

growth notes :

To generate reporter constructs, the following oligonucleotides were used: 5'-CCGGTACCGG GTTGTGGCAG CCAGTCACGT GCCCGCCGCG TAGCCACACC TCTGCTCCTC AGAGCAATGT CAAGCGGTCA CGTGTGATAG CAACAGATCA CGTGGCTGCC ATCGCCCCTC-3' [Oligo A, for wild-type c-MYC binding sites (MBS)1-3]; 5'-ATGAATTCCG GACGTTCTGG GCAGGTGACC GCCACCCATG CGCTGAGGGG CGGACAGGAG GTGCTTCGAC TGGGAGGAGG GCGAAGAGTG TAAGGGGGCG GAGGGGCGAT GGCAGCCAGG-3' [Oligo D, for mutant MBS4]. Different combinations of oligonucleotide pairs (A+B, A+D, C+B, C+D) were annealed and converted to double-stranded fragments through one PCR cycle. These promoter fragments were subcloned into pBV-luc. Further polymerase-derived mutants were identified while sequencing the reporter constructs.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	KpnI
promoter
sequencing_primer_3	LucNrev
sequencing_primer_5	RVprimer3
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MRTL, MYCC, bHLHe39, c-Myc
gene	MYC
id	4609

genbank_ids

mutation

Mutant MBS4

name

c-MYC binding sites

shRNA_sequence

size

species

9606

Homo sapiens