pBV-Luc wt MBS1-4 | Addgene 16564 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pBV-Luc wt MBS1-4

catalog :

16564

citations: 10

Reference
He D, Chen M, Chang L, Gu J, Liu F, Gao X, et al. De novo pyrimidine synthesis fuels glycolysis and confers chemoresistance in gastric cancer. Cancer Lett. 2022;549:215837 pubmed publisher
Soshnikova N, Tatarskiy E, Tatarskiy V, Klimenko N, Shtil A, Nikiforov M, et al. PHF10 subunit of PBAF complex mediates transcriptional activation by MYC. Oncogene. 2021;40:6071-6080 pubmed publisher
Wu M, Chen C, Lin T, Liu Y, Tseng L, Cheng M, et al. Targeting KDM4B that coactivates c-Myc-regulated metabolism to suppress tumor growth in castration-resistant prostate cancer. Theranostics. 2021;11:7779-7796 pubmed publisher
Chang C, Liu H, Chang M, Chang S. Middle East Respiratory Syndrome Coronavirus Nucleocapsid Protein Suppresses Type I and Type III Interferon Induction by Targeting RIG-I Signaling. J Virol. 2020;94: pubmed publisher
Ge Y, Schuster M, Pundhir S, Rapin N, Bagger F, Sidiropoulos N, et al. The splicing factor RBM25 controls MYC activity in acute myeloid leukemia. Nat Commun. 2019;10:172 pubmed publisher
Umbaugh C, Diaz Quiñones A, Neto M, Shearer J, Figueiredo M. A dock derived compound against laminin receptor (37 LR) exhibits anti-cancer properties in a prostate cancer cell line model. Oncotarget. 2018;9:5958-5978 pubmed publisher
Gu Y, Zhang J, Ma X, Kim B, Wang H, Li J, et al. Stabilization of the c-Myc Protein by CAMKII? Promotes T Cell Lymphoma. Cancer Cell. 2017;32:115-128.e7 pubmed publisher
Hahm E, Singh K, Singh S. c-Myc is a novel target of cell cycle arrest by honokiol in prostate cancer cells. Cell Cycle. 2016;15:2309-20 pubmed publisher
Rodrigues P, Macaya I, Bazzocco S, Mazzolini R, Andretta E, Dopeso H, et al. RHOA inactivation enhances Wnt signalling and promotes colorectal cancer. Nat Commun. 2014;5:5458 pubmed publisher
Hermeking H, Rago C, Schuhmacher M, Li Q, Barrett J, Obaya A, et al. Identification of CDK4 as a target of c-MYC. Proc Natl Acad Sci U S A. 2000;97:2229-34 pubmed

product information

Catalog Number :

16564

Product Name :

pBV-Luc wt MBS1-4

article :

doi	10.1073/pnas.050586197
id	1858
pubmed_id	10688915

bacterial resistance :

Ampicillin

cloning :

backbone

pBV-Luc

backbone_mutation

backbone_origin

Vogelstein Lab

backbone_size

4867

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Luciferase

growth notes :

To generate reporter constructs, the following oligonucleotides were used: 5'-CCGGTACCGG GTTGTGGCAG CCAGTCACGT GCCCGCCGCG TAGCCACACC TCTGCTCCTC AGAGCAATGT CAAGCGGTCA CGTGTGATAG CAACAGATCA CGTGGCTGCC ATCGCCCCTC-3' [Oligo A, for wild-type c-MYC binding sites (MBS)1-3]; 5'-ATGAATTCCG GACGTTCTGG GCACGTGACC GCCACCCATG CGCTGAGGGG CGGACAGGAG GTGCTTCGAC TGGGAGGAGG GCGAAGAGTG TAAGGGGGCG GAGGGGCGAT GGCAGCC-3' [Oligo B, for wild-type MBS4]. Different combinations of oligonucleotide pairs (A+B, A+D, C+B, C+D) were annealed and converted to double-stranded fragments through one PCR cycle. These promoter fragments were subcloned into pBV-luc. Further polymerase-derived mutants were identified while sequencing the reporter constructs.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	KpnI
promoter
sequencing_primer_3	LucNrev
sequencing_primer_5	RVprimer3
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MRTL, MYCC, bHLHe39, c-Myc
gene	MYC
id	4609

genbank_ids

mutation

name

c-MYC binding sites

shRNA_sequence

size

species

9606

Homo sapiens